Assessing the effect of dynamics on the closed-loop protein-folding hypothesis

Chintapalli, S. V., Illingworth, C. J.R. , Upton, G. J. G., Sacquin-Mora, S., Reeves, P. J., Mohammedali, H. S. and Reynolds, C. A. (2014) Assessing the effect of dynamics on the closed-loop protein-folding hypothesis. Journal of The Royal Society Interface, 11(91), 20130935. (doi: 10.1098/rsif.2013.0935) (PMID:24258160) (PMCID:PMC3869168)

Abstract

The closed-loop (loop-n-lock) hypothesis of protein folding suggests that loops of about 25 residues, closed through interactions between the loop ends (locks), play an important role in protein structure. Coarse-grain elastic network simulations, and examination of loop lengths in a diverse set of proteins, each supports a bias towards loops of close to 25 residues in length between residues of high stability. Previous studies have established a correlation between total contact distance (TCD), a metric of sequence distances between contacting residues (cf. contact order), and the log-folding rate of a protein. In a set of 43 proteins, we identify an improved correlation (r2 = 0.76), when the metric is restricted to residues contacting the locks, compared to the equivalent result when all residues are considered (r2 = 0.65). This provides qualified support for the hypothesis, albeit with an increased emphasis upon the importance of a much larger set of residues surrounding the locks. Evidence of a similar-sized protein core/extended nucleus (with significant overlap) was obtained from TCD calculations in which residues were successively eliminated according to their hydrophobicity and connectivity, and from molecular dynamics simulations. Our results suggest that while folding is determined by a subset of residues that can be predicted by application of the closed-loop hypothesis, the original hypothesis is too simplistic; efficient protein folding is dependent on a considerably larger subset of residues than those involved in lock formation.

• Item Type: Articles
• Additional Information: We acknowledge support from the University of Essex (S.V.C.) and the Royal Society for a Theo Murphy Blue Skies Award (C.A.R.). C.I. is supported by a Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and the Royal Society (grant number 101239/Z/13/Z). This work was also supported by the Medical Research Council grant number G1001812.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Illingworth, Dr Chris
• Authors: Chintapalli, S. V., Illingworth, C. J.R., Upton, G. J. G., Sacquin-Mora, S., Reeves, P. J., Mohammedali, H. S., and Reynolds, C. A.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
• Journal Name: Journal of The Royal Society Interface
• ISSN: 1742-5689
• ISSN (Online): 1742-5662
• Copyright Holders: Copyright © 2013 The Authors
• First Published: Journal of The Royal Society Interface 11(91):20130935
• Publisher Policy: Reproduced under a Creative Commons License