The antiprotozoal activity of Papua New Guinea propolis and its triterpenes

Alenezi, S. S. et al. (2022) The antiprotozoal activity of Papua New Guinea propolis and its triterpenes. Molecules, 27(5), 1622. (doi: 10.3390/molecules27051622) (PMID:35268726) (PMCID:PMC8911803)

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Profiling a propolis sample from Papua New Guinea (PNG) using high-resolution mass spectrometry indicated that it contained several triterpenoids. Further fractionation by column chromatography and medium-pressure liquid chromatography (MPLC) followed by nuclear magnetic resonance spectroscopy (NMR) identified 12 triterpenoids. Five of these were obtained pure and the others as mixtures of two or three compounds. The compounds identified were: mangiferonic acid, ambonic acid, isomangiferolic acid, ambolic acid, 27-hydroxyisomangiferolic acid, cycloartenol, cycloeucalenol, 24-methylenecycloartenol, 20-hydroxybetulin, betulin, betulinic acid and madecassic acid. The fractions from the propolis and the purified compounds were tested in vitro against Crithidia fasciculata, Trypanosoma congolense, drug-resistant Trypanosoma congolense, Trypanosoma b. brucei and multidrug-resistant Trypanosoma b. brucei (B48). They were also assayed for their toxicity against U947 cells. The compounds and fractions displayed moderate to high activity against parasitic protozoa but only low cytotoxicity against the mammalian cells. The most active isolated compound, 20-hydroxybetulin, was found to be trypanostatic when different concentrations were tested against T. b. brucei growth.

Item Type:Articles
Keywords:Triterpenes, T. brucei, T. congolense, Papua New Guinea, propolis, U947 cells.
Glasgow Author(s) Enlighten ID:Ungogo, Mr Marzuq and Ebiloma, Mr Godwin and De Koning, Professor Harry and Natto, Dr Manal and Igoli, Dr John
Creator Roles:
Ebiloma, G. U.Methodology, Supervision
Natto, M. J.Methodology, Supervision
Ungogo, M. A.Methodology, Supervision
Igoli, J. O.Data curation, Formal analysis, Writing – review and editing
de Koning, H. P.Data curation, Methodology, Formal analysis, Resources, Writing – review and editing, Supervision
Authors: Alenezi, S. S., Alenezi, N. D., Ebiloma, G. U., Natto, M. J., Ungogo, M. A., Igoli, J. O., Ferro, V. A., Gray, A. I., Fearnley, J., de Koning, H. P., and Watson, D. G.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:Molecules
ISSN (Online):1420-3049
Published Online:01 March 2022
Copyright Holders:Copyright © 2022 The Authors
First Published:First published in Molecules 27(5): 1622
Publisher Policy:Reproduced under a Creative Commons License

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