Haney, J., Vijayakrishnan, S. , Streetley, J. , Dee, K., Goldfarb, D. M., Clarke, M. , Mullin, M., Carter, S. D. , Bhella, D. and Murcia, P. R. (2021) Generation of chimeric respiratory viruses with altered tropism by coinfection of influenza A virus and respiratory syncytial virus. bioRxiv, (doi: 10.1101/2021.08.16.456460) (Submitted)
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Abstract
Interactions between co-circulating respiratory viruses are recognized for their impact on viral transmission and clinical outcomes. However, the consequences of these virus-virus interactions at the cellular level are unclear. We coinfected human lung cells with influenza A virus (IAV) and respiratory syncytial virus (RSV). Super-resolution microscopy combined with live-cell imaging and scanning electron microscopy identified extracellular and membrane-associated filamentous structures, likely composed of elements of both IAV and RSV virions. Cryo-electron tomography confirmed the presence of chimeric virus particles exhibiting glycoproteins and ribonucleoproteins of both parental viruses. Functional assays revealed chimeric particles facilitate IAV infection in cells depleted of IAV receptors, demonstrating expanded tropism. Our observations define a previously unknown interaction that is likely to affect virus pathogenesis and have profound implications for infection biology.
| Item Type: | Articles |
|---|---|
| Additional Information: | This work was supported by grants from the Medical Research Council of the United Kingdom (MC_UU_12014/9 to PRM, MR/N013166/1 to JH, MR/R502327/1 to DMG, MC_UU_12014/7 to SV, and MC_UU_12014/7 to DB). The Scottish Centre for Macromolecular Imaging (SCMI) is funded by the MRC (MC_PC_17135) and SFC (H17007). |
| Status: | Submitted |
| Refereed: | No |
| Glasgow Author(s) Enlighten ID: | Carter, Dr Stephen and Clarke, Dr Mairi and Streetley, Dr James and Haney, Joanne and Mullin, Mrs Margaret and Goldfarb, Daniel Max and Bhella, Professor David and Vijayakrishnan, Dr Swetha and Murcia, Professor Pablo and Dee, Dr Kieran |
| Authors: | Haney, J., Vijayakrishnan, S., Streetley, J., Dee, K., Goldfarb, D. M., Clarke, M., Mullin, M., Carter, S. D., Bhella, D., and Murcia, P. R. |
| College/School: | College of Medical Veterinary and Life Sciences College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | bioRxiv |
| Publisher: | Cold Spring Harbor Laboratory |
| Copyright Holders: | Copyright © 2021 The Authors |
| Publisher Policy: | Reproduced under a Creative Commons License |
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