Hulme, H. et al. (2022) Mapping the influence of the gut microbiota on small molecules across the microbiome gut brain axis. Journal of the American Society for Mass Spectrometry, 33(4), pp. 649-659. (doi: 10.1021/jasms.1c00298) (PMID:35262356) (PMCID:PMC9047441)
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Abstract
Microbes exert influence across the microbiome–gut–brain axis through neurotransmitter production, induction of host immunomodulators, or the release or induction of other microbial or host molecules. Here, we used mass spectrometry imaging (MSI), a label-free imaging tool, to map molecular changes in the gut and brain in germ-free, antibiotic-treated and control mice. We determined spatial distribution and relative quantification of neurotransmitters and their precursors in response to the microbiome. Using untargeted MSI, we detected a significant change in the levels of four identified small molecules in the brains of germ-free animals compared to controls. However, antibiotic treatment induced no significant changes in these same metabolites in the brain after 1 week of treatment. This work exemplifies the utility of MSI as a tool for the study of known and discovery of novel, mediators of microbiome-gut-brain axis communication.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Burchmore, Dr Richard and Meikle, Dr Lynsey and Wall, Dr Daniel and Milling, Professor Simon and HULME, Heather |
Authors: | Hulme, H., Meikle, L. M., Strittmatter, N., Swales, J., Hamm, G., Brown, S. L., Milling, S., MacDonald, A. S., Goodwin, R. J. A., Burchmore, R., and Wall, D. M. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Research Centre: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Immunobiology |
Journal Name: | Journal of the American Society for Mass Spectrometry |
Publisher: | American Chemical Society |
ISSN: | 1044-0305 |
ISSN (Online): | 1879-1123 |
Published Online: | 09 March 2022 |
Copyright Holders: | Copyright © 2022 American Society for Mass Spectrometry. |
First Published: | First published in Journal of the American Society for Mass Spectrometry 33(4): 649-659 |
Publisher Policy: | Reproduced in accordance with the publisher copyright policy |
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