Lal, R., Ritchie, J., Richmond, L. and Keeshan, K. (2022) Detecting endogenous TRIB2 protein expression by flow cytometry and western blotting. In: Pseudokinases. Series: Methods in enzymology (667). Academic Press, pp. 59-77. ISBN 9780323915427 (doi: 10.1016/bs.mie.2022.03.024)
Full text not currently available from Enlighten.
Abstract
Protein kinases catalyze the transfer of a phosphate group thereby activating proteins and initiating signaling cascades. Their cousins, the pseudokinases, are enzymatically nonactive counterparts of protein kinases that can be considered zombie enzymes. Interestingly, pseudokinases, which constitute about 10% of the human kinome, have been implicated in many cancers, despite their sequences predicting a lack of catalytic activity. Owing to recent research, it has been demonstrated that dysregulation of many pseudokinases triggers changes in cell signaling, proliferation, and drug resistance. This review is aimed at describing methods that can be used for detection of Tribbles family of pseudokinases, specifically TRIB2. We describe intracellular staining by flow cytometry and Western blotting techniques for the detection of endogenous TRIB2 protein.
| Item Type: | Book Sections |
|---|---|
| Additional Information: | Print ISBN: 9780323915410 |
| Status: | Published |
| Glasgow Author(s) Enlighten ID: | Lal, Dr Ridhima and Keeshan, Dr Karen and Richmond, Dr Laura and Ritchie, Dr Jake |
| Authors: | Lal, R., Ritchie, J., Richmond, L., and Keeshan, K. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences |
| Publisher: | Academic Press |
| ISBN: | 9780323915427 |
| Related URLs: |
University Staff: Request a correction | Enlighten Editors: Update this record
Altmetric
Altmetric