Microalgae show a range of responses to exometabolites of foreign species

Apostolopoulou, N. G., Smeti, E., Lamorgese, M., Varkitzi, I., Whitfield, P., Regnault, C. and Spatharis, S. (2022) Microalgae show a range of responses to exometabolites of foreign species. Algal Research, 62, 102627. (doi: 10.1016/j.algal.2021.102627) (PMID:35311224) (PMCID:PMC8924005)

Abstract

Studies on microalgae interspecific interactions have so far focused either on nutrient competition or allelopathic effects due to excreted substances from Harmful Algal Bloom (HAB) species. Evidence from plants, bacteria and specific microalgae groups, point to a range of responses mediated by sensing or direct chemical impact of exometabolites from foreign species. Such processes remain under-investigated, especially in non-HAB microalgae, despite the importance of such knowledge in ecology and industrial applications. Here, we study the directional effect of exometabolites of 4 “foreign” species Heterosigma akashiwo, Phaeocystis sp., Tetraselmis sp. and Thalassiosira sp. to each of three “target” species across a total of 12 treatments. We disentangle these effects from nutrient competition by adding cell free medium of each “foreign” species into our treatment cultures. We measured the biomass response, to the foreign exometabolites, as cell number and photosynthetic biomass (Chla), whereas nutrient use was measured as residual phosphorus (PO4) and intracellular phosphorus (P). Exometabolites from filtrate of foreign species were putatively annotated by untargeted metabolomics analysis and were discussed in association to observed responses of target species. Among others, these metabolites included L-histidinal, Tiliacorine and dimethylsulfoniopropionate (DMSP). Our findings show that species show a range of responses with the most common being biomass suppression, and less frequent biomass enhancement and intracellular P storage. Filtrate from the green microalgae Tetraselmis caused the most pronounced negative effects suggesting that non-HAB species can also cause negative chemical interference. A candidate metabolite inducing this response is L-histidinal which was measured in high abundance uniquely in Tetraselmis and its L-histidine form derived from bacteria was previously confirmed as a microalgal algicidal. H. akashiwo also induced biomass suppression on other microalgae and a candidate metabolite for this response is Tiliacorine, a plant-derived alkaloid with confirmed cytotoxic activity.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Regnault, Mr Clement and Spatharis, Dr Sofie and Apostolopoulou, Miss Natalia-Georgia

Creator Roles

Apostolopoulou, N. G.: Investigation, Methodology, Formal analysis, Visualization, Writing – original draft
Regnault, C.: Methodology, Investigation, Writing – review and editing
Spatharis, S.: Conceptualization, Methodology, Formal analysis, Visualization, Supervision, Resources, Funding acquisition, Writing – review and editing

• Authors: Apostolopoulou, N. G., Smeti, E., Lamorgese, M., Varkitzi, I., Whitfield, P., Regnault, C., and Spatharis, S.
• College/School: College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Journal Name: Algal Research
• Publisher: Elsevier
• ISSN: 2211-9264
• Published Online: 22 January 2022
• First Published: First published in Algal Research Algal Research 62: 102627
• Publisher Policy: Reproduced under a Creative Commons License

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
306474		Harnessing environmental metabolomics to understand algal warfare	Sofia Spatharis	Wellcome Trust (WELLCOTR)	204820/Z/16/Z	LS - Animal Biology