Identification of a 40S ribosomal protein (S17) that is differentially expressed between the macroschizont and piroplasm stages of Theileria annulata

Oura, C., Kinnaird, J., Tait, A. and Shiels, B. (2002) Identification of a 40S ribosomal protein (S17) that is differentially expressed between the macroschizont and piroplasm stages of Theileria annulata. International Journal for Parasitology, 32(1), pp. 73-80. (doi:10.1016/S0020-7519(01)00305-8) (PMID:11796124)

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Abstract

The nucleotide and protein sequence of the 40S ribosomal protein S17 (RibS17) of the protozoan parasite Theileriaannulata has been determined. Southern blot analysis showed the gene was single copy and comparative sequence analysis revealed that the predicted polypeptide had high sequence homology with the RibS17 from other organisms. Northern blot analysis showed that there was a 3-fold increase in the level of RibS17 RNA between the macroschizont and the piroplasm stage of the lifecycle, whereas, there was no difference in expression between the sporozoite and the macroschizont stages. Antisera to the purified fusion protein, corresponding to the terminal 50 amino acids of the protein sequence, were raised in rabbits. Western analysis detected a polypeptide of the predicted size that was more abundant in the piroplasm stage compared with the macroschizont stage. Immunofluorescence analysis with the same antisera revealed a strong signal in the macroschizont and piroplasm stages, but the antiserum did not cross-react with the bovine host cells. The antisera did, however, cross-react with Toxoplasmagondii tachyzoites and Plasmodiumfalciparum merozoites. The possible functional significance of the stage related increase in abundance of a ribosomal protein is discussed.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Tait, Professor Andy and Shiels, Professor Brian and Kinnaird, Dr Jane
Authors: Oura, C., Kinnaird, J., Tait, A., and Shiels, B.
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:International Journal for Parasitology
Publisher:Elsevier
ISSN:0020-7519
ISSN (Online):1879-0135
Published Online:09 January 2002

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