Three-dimensional tunable fibronectin-collagen platforms for control of cell adhesion and matrix deposition

Asadishekari, M., Mpoyi, E. N., Li, Y., Eslami, J., Walker, M. , Cantini, M. and Gourdon, D. (2022) Three-dimensional tunable fibronectin-collagen platforms for control of cell adhesion and matrix deposition. Frontiers in Physics, 10, 806554. (doi: 10.3389/fphy.2022.806554)

Abstract

The extracellular matrix (ECM) is a complex fibrillar network that couples a cell with its environment and directly regulates cells’ functions via structural, mechanical, and biochemical signals. The goal of this study was to engineer and characterize ECM-mimicking protein platforms with material properties covering both physiological and pathological (tumorous) tissues. We designed and fabricated three-dimensional (3D) fibrillar scaffolds comprising the two major components of the ECM, namely collagen (Col) and fibronectin (Fn), using a previously developed freeze-drying method. While scaffolds porous architecture and mechanics were controlled by varying Col I concentration, Fn deposition and conformation were tuned using varied immersion temperature and assessed via intramolecular Förster Resonance Energy Transfer (FRET). Our data indicate that all scaffolds were able to support various crucial cellular functions such as adhesion, proliferation and matrix deposition. Additionally, we show that, keeping the stiffness constant and tuning the conformation of the Fn layer used to coat the Col scaffolds, we were able to control not only the invasion of cells but also the conformation of the matrix they would deposit, from a compact to an unfolded structure (as observed in the breast tumor microenvironment). Therefore, these tunable scaffolds could be used as 3D cell culture models, in which ECM microarchitecture, mechanics and protein conformation are controlled over large volumes to investigate long-term mechanisms such as wound healing phases and/or vascularization mechanisms in both physiological and pathological (tumorous) microenvironments. These findings have implications for tissue engineering and regenerative medicine.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Gourdon, Professor Delphine and Walker, Dr Matthew and Cantini, Dr Marco
• Authors: Asadishekari, M., Mpoyi, E. N., Li, Y., Eslami, J., Walker, M., Cantini, M., and Gourdon, D.
• College/School: College of Science and Engineering > School of Engineering > Biomedical Engineering
• Journal Name: Frontiers in Physics
• Publisher: Frontiers Research Foundation
• ISSN: 2296-424X
• ISSN (Online): 2296-424X
• Copyright Holders: Copyright © 2022 Asadishekari, Mpoyi, Li, Eslami, Walker, Cantini and Gourdon
• First Published: First published in Frontiers in Physics 10:806554
• Publisher Policy: Reproduced under a Creative Commons licence

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
310302		Manipulating proteins to engineer 3D platforms for cancer research	Delphine Gourdon	The Royal Society (ROYSOC)	RSWF/FT/191020	ENG - Biomedical Engineering