The use of three-dimensional cell culture to study apicobasal polarization and lumen formation

Roman Fernandez, A. , Sandilands, E. and Bryant, D. M. (2022) The use of three-dimensional cell culture to study apicobasal polarization and lumen formation. In: Chang, C. and Wang, J. (eds.) Cell Polarity Signaling. Series: Methods in Molecular Biology (2438). Springer, pp. 439-454. ISBN 9781071620342 (doi: 10.1007/978-1-0716-2035-9_25)

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Abstract

The three-dimensional culture of epithelial cells allows the characterization of processes required for collective epithelial polarization, such as formation of an epithelial lumen. Madin-Darby Canine Kidney (MDCK) cells have been instrumental in pioneering 3-Dimensional culture analysis methods. Here we describe methods for MDCK cell three-dimensional culture, generation of stable engineered cell lines, immunolabeling, and imaging approaches that allow for analysis of apical-basal polarity during lumen formation in this model.

Item Type:Book Sections
Additional Information:This work was supported by a start-up package at the University of Glasgow and support of the Cancer Research UK Beatson Institute (CRUK C596/A17196).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Bryant, Dr David and Roman Fernandez, Mr Alvaro and Sandilands, Dr Emma
Authors: Roman Fernandez, A., Sandilands, E., and Bryant, D. M.
Subjects:Q Science > QH Natural history > QH301 Biology
College/School:College of Medical Veterinary and Life Sciences > School of Cancer Sciences
Publisher:Springer
ISBN:9781071620342
Published Online:11 February 2022

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