2D gel electrophoresis analysis of leishmania proteomes

Naula, C. and Burchmore, R. (2020) 2D gel electrophoresis analysis of leishmania proteomes. In: Michels, P. A. M., Ginger, M. L. and Zilberstein, D. (eds.) Trypanosomatids: Methods and Protocols. Series: Methods in Molecular Biology. Springer US: New York, pp. 577-586. ISBN 9781071602942 (doi: 10.1007/978-1-0716-0294-2_34)

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2D gel electrophoresis enables resolution of intact proteins in complex mixtures and is thus useful for comparative proteomic analysis, particularly of posttranslationally modified proteoforms that might not be distinguished by shotgun proteomic analysis of peptides. 2D gel electrophoresis is a multistep procedure that can require sample-specific optimization. We present a comprehensive protocol that is effective for 2D electrophoretic analysis of proteins from Leishmania promastigotes and may also be employed for Leishmania amastigotes and for trypanosomes.

Item Type:Book Sections
Glasgow Author(s) Enlighten ID:Burchmore, Dr Richard and Naula, Dr Christina
Authors: Naula, C., and Burchmore, R.
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:Methods in Molecular Biology
Publisher:Springer US
Published Online:28 March 2020
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