O'Donovan, L.H., McMonagle, E.L., Taylor, S., Argyle, D.J. and Nicolson, L. (2004) Bioactivity and secretion of interleukin-18 (IL-18) generated by equine and feline IL-18 expression constructs. Veterinary Immunology and Immunopathology, 102(4), pp. 421-428. (doi: 10.1016/j.vetimm.2004.08.003)
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Abstract
Interleukin 18 (IL-18) is a cytokine capable of induction of IFNγ, granulocyte monocyte-colony stimulating factor (GM-CSF), TNFα and IL-1 in immunocompetent cells. Equine and feline plasmid vectors expressing pro-IL-18, mature IL-18 and IL-18 fused to a synthetic signal sequence from human IL-1β receptor antagonist protein (ILRAP), ILRAP-IL-18, have been generated. In vitro protein expression of these constructs was compared by Western blot analysis. These data demonstrated that ILRAP-IL-18 protein was secreted readily from transfected chinese hamster ovary (CHO) cells. A simple bioassay for human IL-18 was recently described using human myelomonocytic KG-1 cells, which produce human IFNγ in response to human IL-18 in a dose dependent manner (Konishi et al., 1997). We demonstrated bioactivity of equine and feline IL-18 protein in transfection products of CHO cells using this assay. Bioactivity of ILRAP-IL-18 protein was demonstrated in the culture medium of transfected CHO cells. These data imply that the ILRAP-IL-18 construct shows potential for use in vivo, where cell secretion of protein is crucial.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | O'Donovan, Dr Lucy and McMonagle, Mrs Linda and Nicolson, Dr Lesley |
Authors: | O'Donovan, L.H., McMonagle, E.L., Taylor, S., Argyle, D.J., and Nicolson, L. |
College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
Journal Name: | Veterinary Immunology and Immunopathology |
Publisher: | Elsevier Inc. |
ISSN: | 0165-2427 |
ISSN (Online): | 1873-2534 |
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