Micromolar metabolite measurement in an electronically multiplexed format

Annese, V. F. , Giagkoulovits, C. , Hu, C. , Al-Rawhani, M. A., Grant, J. , Patil, S. and Cumming, D. (2022) Micromolar metabolite measurement in an electronically multiplexed format. IEEE Transactions on Biomedical Engineering, 69(9), pp. 2715-2722. (doi: 10.1109/TBME.2022.3147855) (PMID:35104208)

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The detection of metabolites such as choline in blood are important in clinical care for patients with cancer and cardiovascular disease. Choline is only present in human blood at low concentrations hence accurate measurement in an affordable point-of-care format is extremely challenging. Integration of microfluidics on to complementary metal-oxide semiconductor (CMOS) technology has the potential to enable advanced sensing technologies with extremely low limit of detection that are well suited to multiple clinical metabolite measurements. Although CMOS and microfluidics are individually mature technologies, their integration has presented challenges that we overcome in a novel, cost-effective, single-step process. To demonstrate the process, we present the microfluidic integration of a metabolomics-on-CMOS point-of-care platform with four capillary microfluidic channels on top of a CMOS optical sensor array. The fabricated device was characterised to verify the required structural profile, mechanical strength, optical spectra, and fluid flow. As a proof of concept, we used the device for the in-vitro quantification of choline in human blood plasma with a limit of detection of 3.2 M and a resolution of 1.6 M.

Item Type:Articles
Additional Information:The authors would like to thank the James Watt Nanofabrication Centre (JWNC), University of Glasgow, and its staff for providing support for the fabrication of the hybrid microfluidics-CMOS device. The research was supported by UKRI grants EP/T00097X and EP/K021966.
Glasgow Author(s) Enlighten ID:Cumming, Professor David and Hu, Dr Chunxiao and Al-Rawhani, Dr Mohammed and Annese, Dr Valerio and Giagkoulovits, Dr Christos and Patil, Dr Samadhan and Grant, Dr James
Authors: Annese, V. F., Giagkoulovits, C., Hu, C., Al-Rawhani, M. A., Grant, J., Patil, S., and Cumming, D.
College/School:College of Science and Engineering > School of Engineering
College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering
Journal Name:IEEE Transactions on Biomedical Engineering
ISSN (Online):1558-2531
Published Online:01 February 2022
Copyright Holders:Copyright © 2022 IEEE
First Published:First published in IEEE Transactions on Biomedical Engineering 69(9): 2715-2722
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
305567QuantIC - The UK Quantum Technoogy Hub in Quantum Enhanced ImagingMiles PadgettEngineering and Physical Sciences Research Council (EPSRC)EP/T00097X/1P&S - Physics & Astronomy
190623The Multi-Corder: Poly-Sensor TechnologyDavid CummingEngineering and Physical Sciences Research Council (EPSRC)EP/K021966/1ENG - Electronics & Nanoscale Engineering