Halstead, S. K., Gourlay, D. and Willison, H. J. (2022) Detection of autoantibodies using combinatorial glycolipid microarrays. In: Kilcoyne, M. and Gerlach, J. Q. (eds.) Glycan Microarrays: Methods and Protocols. Series: Methods in Molecular Biology, 2460. Humana Press: New York, pp. 183-191. ISBN 9781071621479 (doi: 10.1007/978-1-0716-2148-6_11)
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Abstract
Glycolipids cluster in plasma membranes to form heterogenous patches. Whereas lectins and antibodies have been conventionally viewed as binding a single oligosaccharide head group, and assayed accordingly, it is increasingly evident that cis-interactions between glycan headgroups may form unique molecular shapes that either enhance or attenuate binding of antibodies and other proteins. Herein we describe a method for assaying antibody binding to heteromeric glycolipid complexes that allows rapid, simple, inexpensive and high-throughput assessment of binding events, focusing on autoantibodies present in human serum.
| Item Type: | Book Sections |
|---|---|
| Keywords: | Guillain Barré syndrome, Immunoassay, Glycoarray, Glycolipid, Microarray, Ganglioside, Autoimmune disease |
| Status: | Published |
| Glasgow Author(s) Enlighten ID: | Halstead, Dr Susan and Gourlay, Mrs Dawn and Willison, Professor Hugh |
| Authors: | Halstead, S. K., Gourlay, D., and Willison, H. J. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Research Centre: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Immunobiology |
| Publisher: | Humana Press |
| ISSN: | 1940-6029 |
| ISBN: | 9781071621479 |
| Related URLs: |
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