O'Reilly, E. L., Horvatić, A., Kuleš, J., Gelemanović, A., Mrljak, V., Huang, Y., Brady, N., Chadwick, C. C., Eckersall, P. D. and Ridyard, A. (2022) Faecal proteomics in the identification of biomarkers to differentiate canine chronic enteropathies. Journal of Proteomics, 254, 104452. (doi: 10.1016/j.jprot.2021.104452) (PMID:34958965)
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Abstract
Canine chronic enteropathy (CCE) is a collective term used to describe a group of idiopathic enteropathies of dogs that result in a variety of clinical manifestations of intestinal dysfunction. Clinical stratification into food-responsive enteropathy (FRE) or non-food responsive chronic inflammatory enteropathy (CIE), is made retrospectively based on response to treatments. Faecal extracts from those with a FRE (n = 5) and those with non-food responsive chronic inflammatory enteropathies (CIE) (n = 6) were compared to a healthy control group (n = 14) by applying TMT-based quantitative proteomic approach. Many of the proteins with significant differential abundance between groups were pancreatic or intestinal enzymes with pancreatitis-associated protein (identified as REG3α) and pancreatic M14 metallocarboxypeptidase proteins carboxypeptidase A1 and B identified as being of significantly increased abundance in the CCE group. The reactome analysis revealed the recycling of bile acids and salts and their metabolism to be present in the FRE group, suggesting a possible dysbiotic aetiology. Several acute phase proteins were significantly more abundant in the CCE group with the significant increase in haptoglobin in the CIE group especially notable. Further research of these proteins is needed to fully assess their clinical utility as faecal biomarkers for differentiating CCE cases.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Ridyard, Ms Alison and Eckersall, Professor David and Huang, Yixin and Brady, Mrs Nicola and Kules, Mrs Josipa and Horvatic, Ms Anita and O'Reilly, Dr Emily |
| Authors: | O'Reilly, E. L., Horvatić, A., Kuleš, J., Gelemanović, A., Mrljak, V., Huang, Y., Brady, N., Chadwick, C. C., Eckersall, P. D., and Ridyard, A. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
| Journal Name: | Journal of Proteomics |
| Publisher: | Elsevier |
| ISSN: | 1874-3919 |
| ISSN (Online): | 1876-7737 |
| Published Online: | 24 December 2021 |
| Copyright Holders: | Copyright © 2022 Elsevier B.V. |
| First Published: | First published in Journal of Proteomics 254:104452 |
| Publisher Policy: | Reproduced in accordance with the publisher copyright policy |
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