Integrated molecular and behavioural data reveal deep circadian disruption in response to artificial light at night in male Great tits (Parus major)

Dominoni, D. M. et al. (2022) Integrated molecular and behavioural data reveal deep circadian disruption in response to artificial light at night in male Great tits (Parus major). Scientific Reports, 12, 1553. (doi: 10.1038/s41598-022-05059-4) (PMID:35091579) (PMCID:PMC8799718)

[img] Text
262001.pdf - Published Version
Available under License Creative Commons Attribution.



Globally increasing levels of artificial light at night (ALAN) are associated with shifting rhythms of behaviour in many wild species. However, it is unclear whether changes in behavioural timing are paralleled by consistent shifts in the molecular clock and its associated physiological pathways. Inconsistent shifts between behavioural and molecular rhythms, and between different tissues and physiological systems, disrupt the circadian system, which coordinates all major body functions. We therefore compared behavioural, transcriptional and metabolomic responses of captive great tits (Parus major) to three ALAN intensities or to dark nights, recording activity and sampling brain, liver, spleen and blood at mid-day and midnight. ALAN advanced wake-up time, and this shift was paralleled by advanced expression of the clock gene BMAL1 in all tissues, suggesting close links between behaviour and clock gene expression across tissues. However, further analysis of gene expression and metabolites revealed that clock shifts were inconsistent across physiological systems. Untargeted metabolomic profiling showed that only 9.7% of the 755 analysed metabolites followed the behavioural shift. This high level of desynchronization indicates that ALAN disrupted the circadian system on a deep, easily overlooked level. Thus, circadian disruption could be a key mediator of health impacts of ALAN on wild animals.

Item Type:Articles
Additional Information:This work was supported by a Wellcome Trust grant to B.H and D.M.D (097821/Z/11/Z), a Marie-Curie Career Integration Grant to B.H. (ECCIG (618578) Wildclocks), a NERC Highlight Topics grant to D.M.D. (NE/S005773/1) and the Dutch Technology Foundation (STW).
Glasgow Author(s) Enlighten ID:Bellingham, Dr Michelle and Clark, Dr Jessica and Blackburn, Dr Gavin and Helm, Dr Barbara
Authors: Dominoni, D. M., de Jong, M., van Oers, K., O’Shaughnessy, P., Blackburn, G., Atema, E., Mateman, A. C., D’Amelio, P. B., Trost, L., Bellingham, M., Clark, J., Visser, M. E., and Helm, B.
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
Journal Name:Scientific Reports
Publisher:Nature Research
ISSN (Online):2045-2322
Published Online:28 January 2022
Copyright Holders:Copyright © 2022 The Authors
First Published:First published in Scientific Reports 12(1):1553
Publisher Policy:Reproduced under a Creative Commons License

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
303242Unravelling the impact of artificial light at night on circadian disruption, immunity, and infection riskDavide DominoniNatural Environment Research Council (NERC)NE/S005773/1Institute of Biodiversity, Animal Health and Comparative Medicine