Oral cancer theranostic application of FeAu bimetallic nanoparticles conjugated with MMP-1 antibody

Tsai, M.-T., Sun, Y.-S., Keerthi, M., Panda, A. K., Dhawan, U. , Chang, Y.-H., Lai, C.-F., Hsiao, M., Wang, H.-Y. and Chung, R.-J. (2022) Oral cancer theranostic application of FeAu bimetallic nanoparticles conjugated with MMP-1 antibody. Nanomaterials, 12(1), 61. (doi: 10.3390/nano12010061) (PMID:35010011) (PMCID:PMC8746455)

Abstract

Metastatic oral squamous cell carcinoma (SCC) displays a poor disease prognosis with a 5-year survival rate of 39%. Chemotherapy has emerged as the mainstream treatment against small clusters of cancer cells but poses more risks than benefits for metastatic cells due to the non-specificity and cytotoxicity. To overcome these obstacles, we conjugated antibodies specific for matrix metalloproteinase-1 (MMP-1), a prognostic biomarker of SCC, to iron−gold bimetallic nanoparticles (FeAu NPs) and explored the capability of this complex to target and limit SSC cell growth via magnetic field-induced hyperthermia. Our results showed that 4.32 ± 0.79 nm sized FeAu NPs were superparamagnetic in nature with a saturation magnetization (Ms) of 5.8 emu/g and elevated the media temperature to 45 °C, confirming the prospect to deliver hyperthermia. Furthermore, conjugation with MMP-1 antibodies resulted in a 3.07-fold higher uptake in HSC-3 (human tongue squamous cell carcinoma) cells as compared to L929 (fibroblast) cells, which translated to a 5-fold decrease in cell viability, confirming SCC targeting. Finally, upon magnetic stimulation, MMP-1-FeAu NPs conjugate triggered 89% HSC-3 cellular death, confirming the efficacy of antibody-conjugated nanoparticles in limiting SCC growth. The synergistic effect of biomarker-specific antibodies and magnetic nanoparticle-induced hyperthermia may open new doors towards SCC targeting for improved disease prognosis.

• Item Type: Articles
• Additional Information: The authors are grateful for the financial supports of this research by the Ministry of Science and Technology of Taiwan (MOST 110-2223-E-182-001-MY3; MOST 104-2221-E-027-061; MOST 107- 2221-E-027-014; MOST 108-2628-E-027-003-MY3), the National Taipei University of TechnologyMackay Memorial Hospital Joint Research Program (NTUT-MMH-107-04, NTUT-MMH-108-08, NTUT-MMH-109-04).
• Keywords: Oral squamous cell carcinoma, cancer theranostic, iron–gold bimetallic nanoparticles, matrix metalloproteinase-1, magnetic hyperthermia.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Dhawan, Dr Udesh

Creator Roles

Dhawan, U.: Writing – review and editing, Methodology, Formal analysis, Data curation, Visualization

• Authors: Tsai, M.-T., Sun, Y.-S., Keerthi, M., Panda, A. K., Dhawan, U., Chang, Y.-H., Lai, C.-F., Hsiao, M., Wang, H.-Y., and Chung, R.-J.
• College/School: College of Science and Engineering > School of Engineering > Biomedical Engineering
• Journal Name: Nanomaterials
• Publisher: MDPI
• ISSN: 2079-4991
• ISSN (Online): 2079-4991
• Published Online: 27 December 2021
• Copyright Holders: Copyright © 2021 The Authors
• First Published: First published in Nanomaterials 12(1): 61
• Publisher Policy: Reproduced under a Creative Commons License