Docking studies and antiprotozoal activity of secondary metabolites isolated from Scrophularia syriaca Benth. growing in Saudi Arabia

Alkhaldi, A. A.M., Musa, A., Mostafa, E. M., Amin, E. and De Koning, H. P. (2020) Docking studies and antiprotozoal activity of secondary metabolites isolated from Scrophularia syriaca Benth. growing in Saudi Arabia. Records of Natural Products, 14(1), pp. 23-30. (doi: 10.25135/rnp.19.03.1224)

Abstract

Phytochemical study of the ethanolic extract of Scrophularia syriaca Benth. was attained by chromatographic and spectroscopic procedures, which resulted in isolation of eight compounds; 6-O-α-L- rhamnopyranosylcatalpol (1), scropolioside B (2), gmelinoside-L (3), 8-acetyl harpagide (4), scropolioside D (5), scropolioside D2 (6), quercetin (7) and kaempferol-3-O-rutinoside (8). The antiprotozoal activity was evaluated against Trypanosoma brucei brucei (s427-WT), Trypanosoma brucei brucei (TbAT1-B48), Leishmania major and Leishmania mexicana. Compounds 2, 5, 7 and 8 exhibited mild to moderate activities against kinetoplastid parasites compared to pentamidine positive control, the mechanism of antiprotozoal activity was predicted by the molecular docking studies on the target enzyme Trypanosoma brucei glyceraldehyde-3-phosphate dehydrogenase (TbGAPDH).

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: De Koning, Professor Harry
• Authors: Alkhaldi, A. A.M., Musa, A., Mostafa, E. M., Amin, E., and De Koning, H. P.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Records of Natural Products
• Publisher: ACG Publications
• ISSN: 1307-6167
• ISSN (Online): 1307-6167