Materials-driven fibronectin assembly on nanoscale topography enhances mesenchymal stem cell adhesion, protecting cells from bacterial virulence factors and preventing biofilm formation

Damiati, L. A. et al. (2022) Materials-driven fibronectin assembly on nanoscale topography enhances mesenchymal stem cell adhesion, protecting cells from bacterial virulence factors and preventing biofilm formation. Biomaterials, 280, 121263. (doi: 10.1016/j.biomaterials.2021.121263) (PMID:34810036)

Abstract

Post-operative infection is a major complication in patients recovering from orthopaedic surgery. As such, there is a clinical need to develop biomaterials for use in regenerative surgery that can promote mesenchymal stem cell (MSC) osteospecific differentiation and that can prevent infection caused by biofilm-forming pathogens. Nanotopographical approaches to pathogen control are being identified, including in orthopaedic materials such as titanium and its alloys. These topographies use high aspect ratio nanospikes or nanowires to prevent bacterial adhesion but these features also significantly reduce MSC adhesion and activity. Here, we use a poly (ethyl acrylate) (PEA) polymer coating on titanium nanowires to spontaneously organise fibronectin (FN) and to deliver bone morphogenetic protein 2 (BMP2) to enhance MSC adhesion and osteospecific signalling. Using a novel MSC–Pseudomonas aeruginosa co-culture, we show that the coated nanotopographies protect MSCs from cytotoxic quorum sensing and signalling molecules, enhance MSC adhesion and osteoblast differentiation and reduce biofilm formation. We conclude that the PEA polymer-coated nanotopography can both support MSCs and prevent pathogens from adhering to a biomaterial surface, thus protecting from biofilm formation and bacterial infection, and supporting osteogenic repair.

• Item Type: Articles
• Additional Information: L.A.D. was supported by a scholarship from Jeddah University and the Saudi Arabian Government. The work was also supported by grants from EPSRC (EP/K034898/1) and MRC (MR/S010343/1).
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Salmeron-Sanchez, Professor Manuel and Burgess, Dr Karl and Jayawarna, Dr Vineetha and Meek, Professor Dominic and Sprott, Dr Mark and Childs, Dr Peter and Dalby, Professor Matthew and Tsimbouri, Dr Monica and Ramage, Professor Gordon and Damiati, Ms Laila and Llopis-Hernandez, Dr Virginia and Li, Dr Peifeng and Xiao, Yinbo
• Authors: Damiati, L. A., Tsimbouri, M. P., Llopis Hernandez, V., Jayawarna, V., Ginty, M., Childs, P., Xiao, Y., Burgess, K., Wells, J., Sprott, M. R., Meek, R.M. D., Li, P., Oreffo, R. O.C., Nobbs, A., Ramage, G., Su, B., Salmeron-Sanchez, M., and Dalby, M. J.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Medical Veterinary and Life Sciences > School of Molecular Biosciences; College of Medical Veterinary and Life Sciences > School of Life Sciences; College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School; College of Science and Engineering > School of Engineering > Biomedical Engineering; College of Science and Engineering > School of Engineering > Systems Power and Energy
• Journal Name: Biomaterials
• Publisher: Elsevier
• ISSN: 0142-9612
• ISSN (Online): 1878-5905
• Published Online: 17 November 2021
• Copyright Holders: Copyright © 2021 Elsevier Ltd.
• First Published: First published in Biomaterials 280: 121263
• Publisher Policy: Reproduced in accordance with the publisher copyright policy
• Data DOI: 10.5525/gla.researchdata.1051

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
190636		Multiscale topographical modulation of cells and bacteria for next generation orthopaedic implants	Matthew Dalby	Engineering and Physical Sciences Research Council (EPSRC)	EP/K034898/1	Institute of Molecular, Cell & Systems Biology