Gabriel, J. L., Tinti, M., Fuller, W. and Ashford, M. L.J. (2022) Identifying the beta-site amyloid precursor protein cleaving enzyme 1 interactome through the proximity-dependent biotin identification assay. Neuroscience Letters, 767, 136302. (doi: 10.1016/j.neulet.2021.136302) (PMID:34710551)
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Abstract
Beta-site amyloid precursor protein (APP) cleaving enzyme 1 (BACE1) is a key drug target against Alzheimer’s Disease however, due to its promiscuous proteolytic activity, little is known about its physiological functions. Previous studies have analysed BACE1 cleavage products to examine BACE1 interactions and determine substrates, but these studies cannot establish non-enzymatic (and potentially functional) associations. This study used the biotin identification proximity assay to establish the BACE1 interactome in healthy neuronal cells and identified interactions involved in BACE1 trafficking, post-translational modification and substrates. Furthermore, this method has identified a putative novel role for BACE1 in sex hormone signalling and haem regulation through interaction with the progesterone receptor membrane component 2 (PGRC2). Data are available via ProteomeXchange with identifier PXD021464.
| Item Type: | Articles |
|---|---|
| Additional Information: | This work was supported by Alzheimer’s Society (AS DTC-2014-017) and Alzheimer’s Research UK. J.G. was supported by an Alzheimer’s Society Doctoral Training PhD studentship. The contributions of M.Tinti were supported by a Wellcome Trust Investigator Award to Mike A J Ferguson (101842/Z13/Z). |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Fuller, Professor Will |
| Creator Roles: | |
| Authors: | Gabriel, J. L., Tinti, M., Fuller, W., and Ashford, M. L.J. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health |
| Journal Name: | Neuroscience Letters |
| Publisher: | Elsevier |
| ISSN: | 0304-3940 |
| ISSN (Online): | 1872-7972 |
| Published Online: | 25 October 2021 |
| Copyright Holders: | Copyright © 2021 The Authors. |
| First Published: | First published in Neuroscience Letters 767: 136302 |
| Publisher Policy: | Reproduced under a Creative Commons Licence |
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