Officer, L. K., Andreou, K. E., Teodósio, A. V., He, Z. and Le Quesne, J. P. (2020) Automated co-in situ hybridization and immunofluorescence using archival tumor tissue. In: Nielsen, B. S. and Jones, J. (eds.) In Situ Hybridization Protocols. Series: Methods in molecular biology, 2148 (2148). Springer, pp. 245-256. ISBN 9781071606230 (doi: 10.1007/978-1-0716-0623-0_16)
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Abstract
In situ hybridization (ISH) and immunohistochemistry (IHC) are valuable tools for molecular pathology and cancer research. Recent advances in multiplex technology, assay automation, and digital image analysis have enabled the development of co-ISH IHC or immunofluorescence (IF) methods, which allow researchers to simultaneously view and quantify expression of mRNA and protein within the preserved tissue spatial context. These data are vital to the study of the control of gene expression in the complex tumor microenvironment.
Item Type: | Book Sections |
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Additional Information: | Hardcover ISBN: 9781071606223. |
Status: | Published |
Glasgow Author(s) Enlighten ID: | Le Quesne, Professor John |
Authors: | Officer, L. K., Andreou, K. E., Teodósio, A. V., He, Z., and Le Quesne, J. P. |
College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences |
Publisher: | Springer |
ISSN: | 1064-3745 |
ISBN: | 9781071606230 |
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