Kennedy, J. W., Tsimbouri, M. , Campsie, P., Sood, S., Childs, P. G., Reid, S., Young, P. S., Meek, D. R. M., Goodyear, C. S. and Dalby, M. J. (2021) Nanovibrational stimulation inhibits osteoclastogenesis and enhances osteogenesis in co-cultures. Scientific Reports, 11, 22741. (doi: 10.1038/s41598-021-02139-9)
![[img]](https://eprints.gla.ac.uk/style/images/fileicons/text.png) |
Text
258225.pdf - Published Version Available under License Creative Commons Attribution. 6MB |
Abstract
Models of bone remodelling could be useful in drug discovery, particularly if the model is one that replicates bone regeneration with reduction in osteoclast activity. Here we use nanovibrational stimulation to achieve this in a 3D co-culture of primary human osteoprogenitor and osteoclast progenitor cells. We show that 1000 Hz frequency, 40 nm amplitude vibration reduces osteoclast formation and activity in human mononuclear CD14+ blood cells. Additionally, this nanoscale vibration both enhances osteogenesis and reduces osteoclastogenesis in a co-culture of primary human bone marrow stromal cells and bone marrow hematopoietic cells. Further, we use metabolomics to identify Akt (protein kinase C) as a potential mediator. Akt is known to be involved in bone differentiation via transforming growth factor beta 1 (TGFβ1) and bone morphogenetic protein 2 (BMP2) and it has been implicated in reduced osteoclast activity via Guanine nucleotide-binding protein subunit α13 (Gα13). With further validation, our nanovibrational bioreactor could be used to help provide humanised 3D models for drug screening.
| Item Type: | Articles |
|---|---|
| Additional Information: | Funding: This work was funded by The Engineering and Physical Sciences Research Council (grant number EP/N013905/1) & The Royal College of Surgeons Edinburgh (grant number SPPG/17/113). |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Sood, Dr Shatakshi and Dalby, Professor Matthew and Tsimbouri, Dr Monica and Young, Dr Peter and Goodyear, Professor Carl |
| Authors: | Kennedy, J. W., Tsimbouri, M., Campsie, P., Sood, S., Childs, P. G., Reid, S., Young, P. S., Meek, D. R. M., Goodyear, C. S., and Dalby, M. J. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
| Research Centre: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Immunobiology |
| Journal Name: | Scientific Reports |
| Publisher: | Nature Research |
| ISSN: | 2045-2322 |
| ISSN (Online): | 2045-2322 |
| Published Online: | 23 November 2021 |
| Copyright Holders: | Copyright © The Author(s) 2021 |
| First Published: | First published in Scientific Reports 11: 22741 |
| Publisher Policy: | Reproduced under a Creative Commons licence |
| Data DOI: | 10.5525/gla.researchdata.1090 |
University Staff: Request a correction | Enlighten Editors: Update this record
Altmetric
Altmetric