T cell peptides derived from invasive stages of Schistosoma mansoni as potential schistosomiasis vaccine

López-Abán, J., Vicente, B., Kabbas-Piñango, E. , Hernández-Goenaga, J., Sánchez-Montejo, J., Aguiriano, M., del Olmo, E., Vanegas, M., Alfonso Patarroyo, M. and Muro, A. (2021) T cell peptides derived from invasive stages of Schistosoma mansoni as potential schistosomiasis vaccine. Journal of Clinical Medicine, 10(3), 445. (doi: 10.3390/jcm10030445) (PMID:33498845) (PMCID:PMC7865475)

Abstract

Schistosomiasis is a parasitic disease that affects 143 million people in endemic countries. This work analyzed overexpressed sequences from the cercaria phase to the early schistosomulum phase using bioinformatics tools to predict host interaction and selected proteins for predicting T cell epitopes. The final peptides were chemically synthesized, and their toxicity was evaluated in vitro. Peptides were formulated in the Adjuvant Adaptation (ADAD) vaccination system and injected into BALB/c mice that were challenged with S. mansoni cercariae to assess protection and immunogenicity. A total of 39 highly expressed S.mansoni proteins were identified as being of potential interest. Three T cell peptides predicted to bind MHC mouse and human class II were synthesized and formulated for vaccination. SmGSP and SmIKE reduced the number of eggs trapped in the liver by more than 50% in challenged BALB/c mice. The liver of mice vaccinated with either SmGSP or SmTNP had a significantly reduced affected liver surface. Transcriptome-based T cell peptides elicit partial protection and could be candidates for a multiantigen vaccine.

• Item Type: Articles
• Additional Information: This research was funded by RICET RD16/0027/0018 and PI19/01727; Ministerio de Ciencia e Innovación (MICINN) Spain RTI2018-099474-B-I00 and European Union co-financing by FEDER (Una manera de hacer Europa, Sistema Nacional de Garantía Juvenil, Cofinanciación por FEDER, and Iniciativa de Empleo Juvenil BDNS: 427002).
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Kabbas Piñango, Elías

Creator Roles

Kabbas-Piñango, E.: Methodology, Investigation, Writing – original draft, Visualization

• Authors: López-Abán, J., Vicente, B., Kabbas-Piñango, E., Hernández-Goenaga, J., Sánchez-Montejo, J., Aguiriano, M., del Olmo, E., Vanegas, M., Alfonso Patarroyo, M., and Muro, A.
• College/School: College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Journal Name: Journal of Clinical Medicine
• Publisher: MDPI
• ISSN: 2077-0383
• ISSN (Online): 2077-0383
• Published Online: 24 January 2021
• Copyright Holders: Copyright © 2021 The Authors
• First Published: First published in Journal of Clinical Medicine 10(3): 445
• Publisher Policy: Reproduced under a Creative Commons License