Multi-omics approach to elucidate cerebrospinal fluid changes in dogs with intervertebral disc herniation

Horvatić, A. et al. (2021) Multi-omics approach to elucidate cerebrospinal fluid changes in dogs with intervertebral disc herniation. International Journal of Molecular Sciences, 22(21), 11678. (doi: 10.3390/ijms222111678)

Abstract

Herniation of the intervertebral disc (IVDH) is the most common cause of neurological and intervertebral disc degeneration-related diseases. Since the disc starts to degenerate before it can be observed by currently available diagnostic methods, there is an urgent need for novel diagnostic approaches. To identify molecular networks and pathways which may play important roles in intervertebral disc herniation, as well as to reveal the potential features which could be useful for monitoring disease progression and prognosis, multi-omics profiling, including highresolution liquid chromatography-mass spectrometry (LC-MS)-based metabolomics and tandem mass tag (TMT)-based proteomics was performed. Cerebrospinal fluid of nine dogs with IVDH and six healthy controls were used for the analyses, and an additional five IVDH samples were used for proteomic data validation. Furthermore, multi-omics data were integrated to decipher a complex interaction between individual omics layers, leading to an improved prediction model. Together with metabolic pathways related to amino acids and lipid metabolism and coagulation cascades, our integromics prediction model identified the key features in IVDH, namely the proteins follistatin Like 1 (FSTL1), secretogranin V (SCG5), nucleobindin 1 (NUCB1), calcitonin re-ceptorstimulating peptide 2 precursor (CRSP2) and the metabolites N-acetyl-D-glucosamine and adenine, involved in neuropathic pain, myelination, and neurotransmission and inflammatory response, respectively. Their clinical application is to be further investigated. The utilization of a novel integrative interdisciplinary approach may provide new opportunities to apply innovative diagnostic and monitoring methods as well as improve treatment strategies and personalized care for patients with degenerative spinal disorders.

• Item Type: Articles
• Additional Information: Funding: This research was supported by European Commission FP7 “VetMedZg” project (Grant Number 621394) and European Regional Development Fund (Grant Agreement KK.01.1.1.04.0086). The authors declare that there is no conflict of interest.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: McLaughlin, Dr Mark and Eckersall, Professor David
• Authors: Horvatić, A., Gelemanović, A., Pirkić, B., Smolec, O., Ljubić, B. B., Rubić, I., Eckersall, P. D., Mrljak, V., McLaughlin, M., Samardžija, M., and Lipar, M.
• College/School: College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Journal Name: International Journal of Molecular Sciences
• Publisher: MDPI
• ISSN: 1661-6596
• ISSN (Online): 1422-0067
• Published Online: 28 October 2021
• Copyright Holders: Copyright © 2021 by the authors
• First Published: First published in International Journal of Molecular Sciences 22(21): 11678
• Publisher Policy: Reproduced under a Creative Commons licence