Par3 cooperates with Sanpodo for the assembly of Notch clusters following asymmetric division of Drosophila sensory organ precursor cells

Houssin, E., Pinot, M., Bellec, K. and Le Borgne, R. (2021) Par3 cooperates with Sanpodo for the assembly of Notch clusters following asymmetric division of Drosophila sensory organ precursor cells. eLife, 10, e66659. (doi: 10.7554/eLife.66659) (PMID:34596529) (PMCID:PMC8516416)

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Abstract

In multiple cell lineages, Delta-Notch signalling regulates cell fate decisions owing to unidirectional signalling between daughter cells. In Drosophila pupal sensory organ lineage, Notch regulates the intra-lineage pIIa/pIIb fate decision at cytokinesis. Notch and Delta that localise apically and basally at the pIIa-pIIb interface are expressed at low levels and their residence time at the plasma membrane is in the order of minutes. How Delta can effectively interact with Notch to trigger signalling from a large plasma membrane area remains poorly understood. Here, we report that the signalling interface possesses a unique apico-basal polarity with Par3/Bazooka localising in the form of nano-clusters at the apical and basal level. Notch is preferentially targeted to the pIIa-pIIb interface, where it co-clusters with Bazooka and its cofactor Sanpodo. Clusters whose assembly relies on Bazooka and Sanpodo activities are also positive for Neuralized, the E3 ligase required for Delta activity. We propose that the nano-clusters act as snap buttons at the new pIIa-pIIb interface to allow efficient intra-lineage signalling.

Item Type:Articles
Additional Information:This work was supported in part by the ARC (post doctoral fellowship), La Ligue contre le Cancer-Equipe Labellisée (RLB), and the Association Nationale de la Recherche et de la Technologie programme PRC Vie, santé et bien-être CytoSIGN (ANR-16-CE13-004-01 to RLB).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Bellec, Miss Karen
Creator Roles:
Bellec, K.Investigation, Methodology, Resources, Visualization
Authors: Houssin, E., Pinot, M., Bellec, K., and Le Borgne, R.
College/School:College of Medical Veterinary and Life Sciences > School of Cancer Sciences
Journal Name:eLife
Publisher:eLife Sciences Publications
ISSN:2050-084X
ISSN (Online):2050-084X
Copyright Holders:Copyright © 2021 The Authors
First Published:First published in eLife 10:e66659
Publisher Policy:Reproduced under a Creative Commons License

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