Gabrielsen, M., Nagy, L.A., DeLucas, L.J. and Cogdell, R.J. (2010) Self-interaction chromatography as a tool for optimizing conditions for membrane protein crystallization. Acta Crystallographica. Section D: Biological Crystallography, 66(1), pp. 44-50. (doi: 10.1107/S0907444909043972)
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Publisher's URL: http://dx.doi.org/10.1107/S0907444909043972
Abstract
The second virial coefficient, or B value, is a measurement of how well a protein interacts with itself in solution. These interactions can lead to protein crystallization or precipitation, depending on their strength, with a narrow range of B values (the `crystallization slot') being known to promote crystallization. A convenient method of determining the B value is by self-interaction chromatography. This paper describes how the light-harvesting complex 1-reaction centre core complex from Allochromatium vinosum yielded single straight-edged crystals after iterative cycles of self-interaction chromatography and crystallization. This process allowed the rapid screening of small molecules and detergents as crystallization additives. Here, a description is given of how self-interaction chromatography has been utilized to improve the crystallization conditions of a membrane protein.
Item Type: | Articles |
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Keywords: | Crystallization, self-interaction chromatography, B values, light-harvesting complex 1, reaction centre |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Cogdell, Professor Richard and Gabrielsen, Dr Mads |
Authors: | Gabrielsen, M., Nagy, L.A., DeLucas, L.J., and Cogdell, R.J. |
Subjects: | Q Science > QP Physiology |
College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
Journal Name: | Acta Crystallographica. Section D: Biological Crystallography |
Publisher: | Wiley-Blackwell Publishing, Inc. |
ISSN: | 0907-4449 |
ISSN (Online): | 1399-0047 |
Copyright Holders: | Copyright © 2010 International Union of Crystallography |
First Published: | First published in Acta Crystallographica Section D: Biological Crystallography 66(1):44-50 |
Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
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