Removing striping artifacts in light-sheet fluorescence microscopy: a review

Ricci, P., Gavryusev, V., Müllenbroich, C. , Turrini, L., de Vito, G., Silvestri, L., Sancataldo, G. and Pavone, F. S. (2022) Removing striping artifacts in light-sheet fluorescence microscopy: a review. Progress in Biophysics and Molecular Biology, 168, pp. 52-65. (doi: 10.1016/j.pbiomolbio.2021.07.003) (PMID:34274370)

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Abstract

In recent years, light-sheet fluorescence microscopy (LSFM) has found a broad application for imaging of diverse biological samples, ranging from sub-cellular structures to whole animals, both in-vivo and ex-vivo, owing to its many advantages relative to point-scanning methods. By providing the selective illumination of sample single planes, LSFM achieves an intrinsic optical sectioning and direct 2D image acquisition, with low out-of-focus fluorescence background, sample photo-damage and photo-bleaching. On the other hand, such an illumination scheme is prone to light absorption or scattering effects, which lead to uneven illumination and striping artifacts in the images, oriented along the light sheet propagation direction. Several methods have been developed to address this issue, ranging from fully optical solutions to entirely digital post-processing approaches. In this work, we present them, outlining their advantages, performance and limitations.

Item Type:Articles
Additional Information:Funding: This project has received funding from the European Union's Horizon 2020 research and innovation Framework Programme under grant agreements No. 720270 (HBP-SGA1), 785907 (HBPSGA2), 945539 (HBP-SGA3), and 871124 (Laserlab-Europe), and from the EU program H2020 EXCELLENT SCIENCE - European Research Council (ERC) under grant agreement n. 692943 (BrainBIT).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Muellenbroich, Dr Caroline
Authors: Ricci, P., Gavryusev, V., Müllenbroich, C., Turrini, L., de Vito, G., Silvestri, L., Sancataldo, G., and Pavone, F. S.
College/School:College of Science and Engineering > School of Physics and Astronomy
Journal Name:Progress in Biophysics and Molecular Biology
Publisher:Elsevier
ISSN:0079-6107
ISSN (Online):1873-1732
Published Online:15 July 2021
Copyright Holders:Copyright © 2021 The Authors
First Published:First published in Progress in Biophysics and Molecular Biology 168: 52-65
Publisher Policy:Reproduced under a Creative Commons licence

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