Muir, R., Forbes, S., Birch, D. J.S., Vyshemirsky, V. and Rolinski, O. J. (2021) Collagen glycation detected by its intrinsic fluorescence. Journal of Physical Chemistry B, 125(39), pp. 11058-11066. (doi: 10.1021/acs.jpcb.1c05001) (PMID:34555903)
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Abstract
Collagen’s long half-life (in skin approximately 10 years) makes this protein highly susceptible to glycation and formation of the advanced glycation end products (AGEs). Accumulation of cross-linking AGEs in the skin collagen has several detrimental effects; thus, the opportunity for non-invasive monitoring of skin glycation is essential, especially for diabetic patients. In this paper, we report using the time-resolved intrinsic fluorescence of collagen as a biomarker of its glycation. Contrary to the traditional fluorescence intensity decay measurement at the arbitrarily selected excitation and detection wavelengths, we conducted systematic wavelength- and time-resolved measurements to achieve time-resolved emission spectra. Changes in the intrinsic fluorescence kinetics, caused by both collagen aggregation and glycation, have been detected.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Vyshemirsky, Dr Vladislav |
| Authors: | Muir, R., Forbes, S., Birch, D. J.S., Vyshemirsky, V., and Rolinski, O. J. |
| College/School: | College of Science and Engineering > School of Mathematics and Statistics > Statistics |
| Journal Name: | Journal of Physical Chemistry B |
| Publisher: | American Chemical Society |
| ISSN: | 1520-6106 |
| ISSN (Online): | 1520-5207 |
| Published Online: | 24 September 2021 |
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