Ribosome-associated vesicles: a dynamic subcompartment of the endoplasmic reticulum in secretory cells

Carter, S. D. et al. (2020) Ribosome-associated vesicles: a dynamic subcompartment of the endoplasmic reticulum in secretory cells. Science Advances, 6(14), eaay9572. (doi: 10.1126/sciadv.aay9572) (PMID:32270040) (PMCID:PMC7112762)

Abstract

The endoplasmic reticulum (ER) is a highly dynamic network of membranes. Here, we combine live-cell microscopy with in situ cryo–electron tomography to directly visualize ER dynamics in several secretory cell types including pancreatic β-cells and neurons under near-native conditions. Using these imaging approaches, we identify a novel, mobile form of ER, ribosome-associated vesicles (RAVs), found primarily in the cell periphery, which is conserved across different cell types and species. We show that RAVs exist as distinct, highly dynamic structures separate from the intact ER reticular architecture that interact with mitochondria via direct intermembrane contacts. These findings describe a new ER subcompartment within cells.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Carter, Dr Stephen
• Authors: Carter, S. D., Hampton, C. M., Langlois, R., Melero, R., Farino, Z. J., Calderon, M. J., Li, W., Wallace, C. T., Tran, N. H., Grassucci, R. A., Siegmund, S. E., Pemberton, J., Morgenstern, T. J., Eisenman, L., Aguilar, J. I., Greenberg, N. L., Levy, E. S., Yi, E., Mitchell, W. G., Rice, W. J., Wigge, C., Pilli, J., George, E. W., Aslanoglou, D., Courel, M., Freyberg, R. J., Javitch, J. A., Wills, Z. P., Area-Gomez, E., Shiva, S., Bartolini, F., Volchuk, A., Murray, S. A., Aridor, M., Fish, K. N., Walter, P., Balla, T., Fass, D., Wolf, S. G., Watkins, S. C., Carazo, J. M., Jensen, G. J., Frank, J., and Freyberg, Z.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
• Journal Name: Science Advances
• Publisher: American Association for the Advancement of Science
• ISSN: 2375-2548
• ISSN (Online): 2375-2548
• Copyright Holders: Copyright © 2020 The Authors
• First Published: First published in Science Advances 6(14): eaay9572
• Publisher Policy: Reproduced under a Creative Commons License