Uncovering viral RNA-host cell interactions on a proteome-wide scale

Iselin, L., Palmalux, N., Kamel, W. , Simmonds, P., Mohammed, S. and Castello, A. (2022) Uncovering viral RNA-host cell interactions on a proteome-wide scale. Trends in Biochemical Sciences, 47(1), pp. 23-38. (doi: 10.1016/j.tibs.2021.08.002) (PMID:34509361)

[img] Text
249270.pdf - Published Version
Available under License Creative Commons Attribution.



RNA viruses interact with a wide range of cellular RNA-binding proteins (RBPs) during their life cycle. The prevalence of these host–virus interactions has been highlighted by new methods that elucidate the composition of viral ribonucleoproteins (vRNPs). Applied to 11 viruses so far, these approaches have revealed hundreds of cellular RBPs that interact with viral (v)RNA in infected cells. However, consistency across methods is limited, raising questions about methodological considerations when designing and interpreting these studies. Here, we discuss these caveats and, through comparing available vRNA interactomes, describe RBPs that are consistently identified as vRNP components and outline their potential roles in infection. In summary, these novel approaches have uncovered a new universe of host–virus interactions holding great therapeutic potential.

Item Type:Articles
Additional Information:A.C. is supported by Medical Research Council (MRC) Career Development Award MR/L019434/1 and MRC grants MR/R021562/1, MC_UU_12014/10, and MC_UU_12014/12. L.I. is funded by BBSRC DTP scholarship number BB/M011224/1. N.P. is funded by the MRC CVR PhD programme. W.K. is funded by the European Union’s Horizon 2020 research and innovation programme under Marie-Skłodowska-Curie actions (grant 842067).
Glasgow Author(s) Enlighten ID:Palmalux, Natasha and Castello, Professor Alfredo and Iselin, Louisa and Kamel, Dr Wael
Authors: Iselin, L., Palmalux, N., Kamel, W., Simmonds, P., Mohammed, S., and Castello, A.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
Journal Name:Trends in Biochemical Sciences
Publisher:Elsevier (Cell Press)
ISSN (Online):1362-4326
Published Online:09 September 2021
Copyright Holders:Copyright © 2021 The Authors
First Published:First published in Trends in Biochemical Sciences 47(1): 23-38
Publisher Policy:Reproduced under a Creative Commons licence

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
Innate Immunity and Host Species BarriersMassimo PalmariniMedical Research Council (MRC)MC_UU_12014/10III - Centre for Virus Research
Viral Genomics and BioinformaticsAndrew DavisonMedical Research Council (MRC)MC_UU_12014/12III-MRC-GU Centre for Virus Research