Magnetic resonance imaging (MRI) of heavy-metal transport and fate in an artificial biofilm

Phoenix, V.R. , Holmes, W.M. and Ramanan, B. (2008) Magnetic resonance imaging (MRI) of heavy-metal transport and fate in an artificial biofilm. Mineralogical Magazine, 72(1), pp. 483-486. (doi: 10.1180/minmag.2008.072.1.483)

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Publisher's URL: http://dx.doi.org/10.1180/minmag.2008.072.1.483

Abstract

Unlike planktonic systems, reaction rates in biofilms are often limited by mass transport, which controls the rate of supply of contaminants into the biofilm matrix. To help understand this phenomenon, we investigated the potential of magnetic resonance imaging (MRI) to spatially quantify copper transport and fate in biofilms. For this initial study we utilized an artificial biofilm composed of a 50:50 mix of bacteria and agar. MRI successfully mapped Cu2+ uptake into the artificial biofilm by mapping T2 relaxation rates. A calibration protocol was used to convert T2 values into actual copper concentrations. Immobilization rates in the artificial biofilm were slow compared to the rapid equilibration of planktonic systems. Even after 36 h, the copper front had migrated only 3 mm into the artificial biofilm and at this distance from the copper source, concentrations were very low. This slow equilibration is a result of (1) the time it takes copper to diffuse over such distances and (2) the adsorption of copper onto cell surfaces, which further impedes copper diffusion. The success of this trial run indicates MRI could be used to quantitatively map heavy metal transport and immobilization in natural biofilms.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Holmes, Dr William and Phoenix, Dr Vernon
Authors: Phoenix, V.R., Holmes, W.M., and Ramanan, B.
Subjects:Q Science > QR Microbiology
Q Science > QC Physics
College/School:College of Medical Veterinary and Life Sciences > School of Psychology & Neuroscience
College of Science and Engineering > School of Geographical and Earth Sciences
Journal Name:Mineralogical Magazine
ISSN:0026-461X
ISSN (Online):1471-8022
Copyright Holders:Copyright © 2008 Mineralogical Society
First Published:First published in Mineralogical Magazine 72(1):483-486
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher.

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