Milk and serum proteomes in subclinical and clinical mastitis in Simmental cows

Turk, R. et al. (2021) Milk and serum proteomes in subclinical and clinical mastitis in Simmental cows. Journal of Proteomics, 244, 104277. (doi: 10.1016/j.jprot.2021.104277) (PMID:34044168)

Abstract

Bovine mastitis causes changes in the milk and serum proteomes. Here changes in both proteomes caused by naturally occurring subclinical and clinical mastitis have been characterised and quantified. Milk and serum samples from healthy dairy cows (n = 10) were compared to those of cows with subclinical (n = 12) and clinical mastitis (n = 10) using tandem mass tag (TMT) proteomics. Proteins that significantly increased or decreased in milk (n = 237) or serum (n = 117) were quantified and classified by the type of change in subclinical and clinical mastitis. A group of the proteins (n = 38) showed changes in both milk and serum a number of which decreased in the serum but increased in milk, suggesting a particular role in host defence for maintaining and restoring homeostasis during the disease. Proteins affected by bovine mastitis included proteins in host defence and coagulation pathways. Investigation of the modified proteomes in milk and serum was assessed by assays for haptoglobin, serum amyloid A and α acid glycoprotein validating the results obtained by quantitative proteomics. Alteration of abundance patterns of milk and serum proteins, together with pathway analysis reveal multiple interactions related to proteins affected by mastitis. Data are available via ProteomeXchange with identifier PXD022595. SIGNIFICANCE: Mastitis is the most serious condition to affect dairy cows and leads to reduced animal welfare as well as having a negative economic effect for the dairy industry. Proteomics has previously identified changes in abundance of milk proteins during mastitis, but there have been few investigations addressing changes that may affect proteins in the blood during the infection. In this study, changes in the abundance of proteins of milk and serum, caused by naturally occurring mastitis have been characterised by proteomics using a quantitative approach and both subclinical and clinical cases of mastitis have been investigated. In both milk and serum, change in individual proteins was determined and classified into varying types of altering abundance, such as increasing in subclinical mastitis, but showing no further increase in clinical mastitis. Of special interest were the proteins that altered in abundance in both milk and serum which either showed similar trends - increasing or decreasing in both biological fluids or showed reciprocal change decreasing in serum but increasing in milk. As well as characterising proteins as potential markers of mastitis and the severity of the disease, these results provide insight into the pathophysiology of the host response to bovine mastitis.

• Item Type: Articles
• Additional Information: The proteomic analysis was supported by the European Commission FP7 “VetMedZg” project (grant number 621394) and European Regional Development Fund (Grant Agreement KK.01.1.1.04.0086).
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Eckersall, Professor David and Horvatic, Ms Anita and Kules, Mrs Josipa

Creator Roles

Kules, J.: Investigation, Methodology, Formal analysis, Visualization, Writing – original draft, Writing – review and editing
Horvatic, A.: Investigation, Methodology, Formal analysis, Data curation, Writing – original draft, Writing – review and editing
Eckersall, D.: Conceptualization, Data curation, Supervision, Funding acquisition, Writing – original draft, Writing – review and editing

• Authors: Turk, R., Rošić, N., Kules, J., Horvatic, A., Gelemanovic, A., Galen, A., Ljubić, B. B., Benić, M., Stevanović, V., Mrljak, V., Chadwick, C. C., and Eckersall, P. D.
• College/School: College of Medical Veterinary and Life Sciences; College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Journal Name: Journal of Proteomics
• Publisher: Elsevier
• ISSN: 1874-3919
• ISSN (Online): 1874-3919
• Published Online: 24 May 2021
• Copyright Holders: Copyright © 2021 The Authors
• First Published: First published in Journal of Proteomics 244:104277
• Publisher Policy: Reproduced under a Creative Commons License