Pre-existing hypertension and pregnancy induced hypertension reveal molecular differences in placental proteome in rodents

Mary, S. , Small, H., Herse, F., Carrick, E., Flynn, A., Mullen, W. , Dechend, R. and Delles, C. (2021) Pre-existing hypertension and pregnancy induced hypertension reveal molecular differences in placental proteome in rodents. Physiological Genomics, 53(6), pp. 259-268. (doi: 10.1152/physiolgenomics.00160.2020) (PMID:33969702)

[img] Text
240794.pdf - Accepted Version

4MB
[img] Text
240794Suppl.pdf - Supplemental Material

2MB

Abstract

Pre-existing or new onset of hypertension affects pregnancy and is one of the leading causes of maternal and fetal morbidity and mortality. In certain cases, it also leads to long term maternal cardiovascular complications. The placenta is a key player in the pathogenesis of complicated hypertensive pregnancies, however the pathomechanisms leading to an abnormal placenta are poorly understood. In this study we compared the placental proteome of two rat models: a pre-existing hypertension pregnancy model (stroke-prone spontaneously hypertensive, SHRSP) and the transgenic RAS activated gestational hypertension model (transgenic for human angiotensinogen Sprague-Dawley rats, SD-PE). Label-free proteomics using nano LC-MS/MS was performed for identification and quantification of proteins. Between the two models, we found widespread differences in the expression of placental proteins including those related to hypertension, inflammation and trophoblast invasion; whereas pathways such as regulation of serine endopeptidase activity, tissue injury response, coagulation and complement activation were enriched in both models. We present for the first time the placental proteome of SHRSP and SD-PE and provide insight into the molecular make-up of models of hypertensive pregnancy. Our study informs future research into specific preeclampsia and chronic hypertension pregnancy mechanisms and translation of rodent data to the clinic.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Mullen, Dr Bill and Small, Dr Heather and Samji, Dr Sheon and Flynn, Mr Arun and Carrick, Dr Emma and Delles, Professor Christian
Authors: Mary, S., Small, H., Herse, F., Carrick, E., Flynn, A., Mullen, W., Dechend, R., and Delles, C.
College/School:College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
College of Science and Engineering > School of Chemistry
Journal Name:Physiological Genomics
Publisher:American Physiological Society
ISSN:1094-8341
ISSN (Online):1531-2267
Published Online:14 June 2021
Copyright Holders:Copyright © 2021 American Physiological Society
First Published:First published in Physiological Genomics 53(6): 259-268
Publisher Policy:Reproduced in accordance with the publisher copyright policy

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
303944BHF Centre of ExcellenceRhian TouyzBritish Heart Foundation (BHF)RE/18/6/34217CAMS - Cardiovascular Science