Koehnke, J. , Katsamba, P. S., Ahlsen, G., Bahna, F., Vendome, J., Honig, B., Shapiro, L. and Jin, X. (2010) Splice form dependence of β-neurexin/neuroligin binding interactions. Neuron, 67(1), pp. 61-74. (doi: 10.1016/j.neuron.2010.06.001) (PMID:20624592) (PMCID:PMC2910870)
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Abstract
Alternatively spliced β-neurexins (β-NRXs) and neuroligins (NLs) are thought to have distinct extracellular binding affinities, potentially providing a β-NRX/NL synaptic recognition code. We utilized surface plasmon resonance to measure binding affinities between all combinations of alternatively spliced β-NRX 1-3 and NL 1-3 ectodomains. Binding was observed for all β-NRX/NL pairs. The presence of the NL1 B splice insertion lowers β-NRX binding affinity by ∼2-fold, while β-NRX splice insertion 4 has small effects that do not synergize with NL splicing. New structures of glycosylated β-NRXs 1 and 2 containing splice insertion 4 reveal that the insertion forms a new β strand that replaces the β10 strand, leaving the NL binding site intact. This helps to explain the limited effect of splice insert 4 on NRX/NL binding affinities. These results provide new structural insights and quantitative binding information to help determine whether and how splice isoform choice plays a role in β-NRX/NL-mediated synaptic recognition.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Koehnke, Professor Jesko |
Authors: | Koehnke, J., Katsamba, P. S., Ahlsen, G., Bahna, F., Vendome, J., Honig, B., Shapiro, L., and Jin, X. |
College/School: | College of Science and Engineering > School of Chemistry |
Journal Name: | Neuron |
Publisher: | Cell Press |
ISSN: | 0896-6273 |
ISSN (Online): | 1097-4199 |
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