The cyanobactin heterocyclase enzyme: a processive adenylase that operates with a defined order of reaction

Koehnke, J. et al. (2013) The cyanobactin heterocyclase enzyme: a processive adenylase that operates with a defined order of reaction. Angewandte Chemie (International Edition), 52(52), pp. 13991-13996. (doi: 10.1002/anie.201306302) (PMID:24214017) (PMCID:PMC3995012)

Abstract

Counting backwards: The cyanobactin class of heterocyclases, exemplified by TruD, possess an almost unique combination of processivity, specificity, chemical versatility, and promiscuity. TruD is shown by biochemical assay to be an adenylase, and processes cysteines in a defined order. The entire substrate leader can be removed and TruD will process a single specific cysteine residue; however the role of leader is to permit processivity through a balance of recognition. ATP/AMP=adenosine tri/monophosphate; PPi=pyrophosphate.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Koehnke, Professor Jesko
• Authors: Koehnke, J., Bent, A. F., Zollman, D., Smith, K., Houssen, W. E., Zhu, X., Mann, G., Lebl, T., Scharff, R., Shirran, S., Botting, C. H., Jaspars, M., Schwarz‐Linek, U., and Naismith, J. H.
• College/School: College of Science and Engineering > School of Chemistry
• Journal Name: Angewandte Chemie (International Edition)
• Publisher: Wiley
• ISSN: 1433-7851
• ISSN (Online): 1521-3773
• Copyright Holders: Copyright © 2013 The Authors
• First Published: First published in Angewandte Chemie (International Edition) 52(52): 13991 –13996
• Publisher Policy: Reproduced under a Creative Commons licence