The cyanobactin heterocyclase enzyme: a processive adenylase that operates with a defined order of reaction

Koehnke, J. et al. (2013) The cyanobactin heterocyclase enzyme: a processive adenylase that operates with a defined order of reaction. Angewandte Chemie (International Edition), 52(52), pp. 13991-13996. (doi: 10.1002/anie.201306302) (PMID:24214017) (PMCID:PMC3995012)

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Abstract

Counting backwards: The cyanobactin class of heterocyclases, exemplified by TruD, possess an almost unique combination of processivity, specificity, chemical versatility, and promiscuity. TruD is shown by biochemical assay to be an adenylase, and processes cysteines in a defined order. The entire substrate leader can be removed and TruD will process a single specific cysteine residue; however the role of leader is to permit processivity through a balance of recognition. ATP/AMP=adenosine tri/monophosphate; PPi=pyrophosphate.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Koehnke, Professor Jesko
Authors: Koehnke, J., Bent, A. F., Zollman, D., Smith, K., Houssen, W. E., Zhu, X., Mann, G., Lebl, T., Scharff, R., Shirran, S., Botting, C. H., Jaspars, M., Schwarz‐Linek, U., and Naismith, J. H.
College/School:College of Science and Engineering > School of Chemistry
Journal Name:Angewandte Chemie (International Edition)
Publisher:Wiley
ISSN:1433-7851
ISSN (Online):1521-3773
Copyright Holders:Copyright © 2013 The Authors
First Published:First published in Angewandte Chemie (International Edition) 52(52): 13991 –13996
Publisher Policy:Reproduced under a Creative Commons licence

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