The structure of the cyanobactin domain of unknown function from PatG in the patellamide gene cluster

Mann, G., Koehnke, J. , Bent, A. F., Graham, R., Houssen, W., Jaspars, M., Schwarz-Linek, U. and Naismith, J. H. (2014) The structure of the cyanobactin domain of unknown function from PatG in the patellamide gene cluster. Acta Crystallographica. Section F: Structural Biology Communications, 70, pp. 1597-1603. (doi: 10.1107/s2053230x1402425x) (PMID:25484206) (PMCID:PMC4259220)

Abstract

Patellamides are members of the cyanobactin family of ribosomally synthesized and post-translationally modified cyclic peptide natural products, many of which, including some patellamides, are biologically active. A detailed mechanistic understanding of the biosynthetic pathway would enable the construction of a biotechnological `toolkit' to make novel analogues of patellamides that are not found in nature. All but two of the protein domains involved in patellamide biosynthesis have been characterized. The two domains of unknown function (DUFs) are homologous to each other and are found at the C-termini of the multi-domain proteins PatA and PatG. The domain sequence is found in all cyanobactin-biosynthetic pathways characterized to date, implying a functional role in cyanobactin biosynthesis. Here, the crystal structure of the PatG DUF domain is reported and its binding interactions with plausible substrates are investigated.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Koehnke, Professor Jesko
• Authors: Mann, G., Koehnke, J., Bent, A. F., Graham, R., Houssen, W., Jaspars, M., Schwarz-Linek, U., and Naismith, J. H.
• College/School: College of Science and Engineering > School of Chemistry
• Journal Name: Acta Crystallographica. Section F: Structural Biology Communications
• Publisher: International Union of Crystallography
• ISSN: 2053-230X
• ISSN (Online): 2053-230X
• Copyright Holders: Copyright © 2014 The Authors
• First Published: First published in Acta Crystallographica. Section F: Structural Biology Communications F70:1597-1603
• Publisher Policy: Reproduced under a Creative Commons licence