Hill, E., Clarke, M. and Barr, F. A. (2000) The Rab6-binding kinesin, Rab6-KIFL, is required for cytokinesis. EMBO Journal, 19(21), pp. 5711-5719. (doi: 10.1093/emboj/19.21.5711) (PMID:11060022) (PMCID:PMC305783)
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Abstract
The Rab6‐binding kinesin, Rab6‐KIFL, was identified in a two‐hybrid screen for proteins that interact with Rab6, a small GTPase involved in membrane traffic through the Golgi apparatus. We find that Rab6‐KIFL accumulates in mitotic cells where it localizes to the midzone of the spindle during anaphase, and to the cleavage furrow and midbody during telophase. Overexpression of Rab6‐KIFL causes a cell division defect resulting in cell death. Microinjection of antibodies to Rab6‐KIFL results in the cells becoming binucleate after one cell cycle, and time‐lapse microscopy reveals that this is due to a defect in cleavage furrow formation and thus cytokinesis. These data show that endogenous Rab6‐KIFL functions in cell division during cleavage furrow formation and cytokinesis, in addition to its previously described role in membrane traffic.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Clarke, Dr Mairi |
| Authors: | Hill, E., Clarke, M., and Barr, F. A. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | EMBO Journal |
| Publisher: | EMBO Press |
| ISSN: | 0261-4189 |
| ISSN (Online): | 1460-2075 |
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