Structure and assembly of the Ebola virus nucleocapsid

Wan, W., Kolesnikova, L., Clarke, M. , Koehler, A., Noda, T., Becker, S. and Briggs, J. A.G. (2017) Structure and assembly of the Ebola virus nucleocapsid. Nature, 551(7680), pp. 394-397. (doi: 10.1038/nature24490) (PMID:29144446) (PMCID:PMC5714281)

Full text not currently available from Enlighten.


Ebola and Marburg viruses are filoviruses: filamentous, enveloped viruses that cause haemorrhagic fever1. Filoviruses are within the order Mononegavirales2, which also includes rabies virus, measles virus, and respiratory syncytial virus. Mononegaviruses have non-segmented, single-stranded negative-sense RNA genomes that are encapsidated by nucleoprotein and other viral proteins to form a helical nucleocapsid. The nucleocapsid acts as a scaffold for virus assembly and as a template for genome transcription and replication. Insights into nucleoprotein–nucleoprotein interactions have been derived from structural studies of oligomerized, RNA-encapsidating nucleoprotein3,4,5,6, and cryo-electron microscopy of nucleocapsid7,8,9,10,11,12 or nucleocapsid-like structures11,12,13. There have been no high-resolution reconstructions of complete mononegavirus nucleocapsids. Here we apply cryo-electron tomography and subtomogram averaging to determine the structure of Ebola virus nucleocapsid within intact viruses and recombinant nucleocapsid-like assemblies. These structures reveal the identity and arrangement of the nucleocapsid components, and suggest that the formation of an extended α-helix from the disordered carboxy-terminal region of nucleoprotein-core links nucleoprotein oligomerization, nucleocapsid condensation, RNA encapsidation, and accessory protein recruitment.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Clarke, Dr Mairi
Authors: Wan, W., Kolesnikova, L., Clarke, M., Koehler, A., Noda, T., Becker, S., and Briggs, J. A.G.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
Journal Name:Nature
Publisher:Nature Research
ISSN (Online):1476-4687
Published Online:08 November 2017

University Staff: Request a correction | Enlighten Editors: Update this record