Modeling human hepatic steatosis in pluripotent stem cell-derived hepatocytes

Sinton, M. C. , Meseguer-Ripolles, J., Lucendo-Villarin, B., Drake, A. J. and Hay, D. C. (2021) Modeling human hepatic steatosis in pluripotent stem cell-derived hepatocytes. STAR Protocols, 2(2), 100493. (doi: 10.1016/j.xpro.2021.100493) (PMID:33997813) (PMCID:PMC8091923)

Abstract

This protocol describes the production of hepatocyte-like cells (HLCs) from human pluripotent stem cells and how to induce hepatic steatosis, a condition characterized by intracellular lipid accumulation. Following differentiation to an HLC phenotype, intracellular lipid accumulation is induced with a steatosis induction cocktail, allowing the user to examine the cellular processes that underpin hepatic steatosis. Furthermore, the renewable nature of our system, on a defined genetic background, permits in-depth mechanistic analysis, which may facilitate therapeutic target identification in the future. For complete details on the use and execution of this protocol, please refer to Sinton et al. (2021).

• Item Type: Articles
• Additional Information: M.C.S. was supported by a British Heart Foundation PhD studentship (FS/16/54/32730) and the British Heart Foundation Centre of Research Excellence. This work was supported in part by the Wellcome Trust [grant number 208400/Z/17/Z]. D.C.H., J.M.R., and B.L.V. were supported with awards from the MRC Doctoral Training Partnership (MR/K501293/1) and the Chief Scientist Office (TCS/16/37). A.J.D. was funded by the British Heart Foundation Centre of Research Excellence, University of Edinburgh.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Sinton, Dr Matthew

Creator Roles

Sinton, M. C.: Conceptualization, Methodology, Validation, Formal analysis, Investigation, Data curation, Writing – original draft, Writing – review and editing, Visualization

• Authors: Sinton, M. C., Meseguer-Ripolles, J., Lucendo-Villarin, B., Drake, A. J., and Hay, D. C.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: STAR Protocols
• Publisher: Cell Press
• ISSN: 2666-1667
• ISSN (Online): 2666-1667
• Published Online: 21 April 2021
• Copyright Holders: Copyright © 2021 The Author(s)
• First Published: First published in STAR Protocols 2(2):100493
• Publisher Policy: Reproduced under a Creative Commons Licence