An illustrative analysis of atypical gas production profiles obtained from in vitro digestibility studies using fecal inoculum

Dhanoa, M. S., López, S., Powell, C. D., Sanderson, R., Ellis, J. L., Murray, J.-A. D. , Garber, A. , Williams, B. A. and France, J. (2021) An illustrative analysis of atypical gas production profiles obtained from in vitro digestibility studies using fecal inoculum. Animals, 11(4), 1069. (doi: 10.3390/ani11041069) (PMID:33918882) (PMCID:PMC8069660)

Abstract

Gas production profiles typically show a monotonically increasing monophasic pattern. However, atypical gas production profiles exist whereby at least two consecutive phases of gas production or additional extraneous features that distort the typical profile are present. Such profiles are more likely to occur with the use of a fecal inoculum and are much less well described. The presence of multiple phases or non-descript extraneous features makes it difficult to apply directly recommended modeling approaches such as standard response functions or classical growth functions. To overcome such difficulties, extensions of the Mitscherlich equation and a numerical modeling option also based on the Mitscherlich are explored. The numerical modeling option uses an estimate of relative rate obtained from the smoothed data profile and an estimate of maximum gas produced together with any lag time information drawn from the raw data to construct a simple Mitscherlich equation. In summary, this article illustrates the analysis of atypical gas production profiles obtained using a fecal inoculum and explores the methodology of numerical modeling to reconstruct equivalent typical growth-like trends.

• Item Type: Articles
• Additional Information: This research was funded in part by The Canada Research Chairs program, grant number 045867 (Natural Sciences and Engineering Research Council of Canada, Ottawa).
• Keywords: Gas production technique, feedstuff evaluation, Mitscherlich equation, atypical profiles, numerical modeling, equivalent profile construction.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Garber, Dr Anna and Murray, Professor Jo-Anne

Creator Roles

Murray, J.-A. D.: Resources, Writing – review and editing
Garber, A.: Resources, Writing – review and editing

• Authors: Dhanoa, M. S., López, S., Powell, C. D., Sanderson, R., Ellis, J. L., Murray, J.-A. D., Garber, A., Williams, B. A., and France, J.
• College/School: College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Journal Name: Animals
• Publisher: MDPI
• ISSN: 2076-2615
• ISSN (Online): 2076-2615
• Published Online: 09 April 2021
• Copyright Holders: Copyright © 2021 The Authors
• First Published: First published in Animals 11(4): 1069
• Publisher Policy: Reproduced under a Creative Commons License