Chisholm, K. I., Lo Re, L., Polgár, E., Gutierrez-Mecinas, M. , Todd, A. J. and McMahon, S. B. (2021) Encoding of cutaneous stimuli by lamina I projection neurons. Pain, 162(9), pp. 2405-2417. (doi: 10.1097/j.pain.0000000000002226) (PMID:33769365) (PMCID:PMC8374708)
Text
238397.pdf - Published Version Available under License Creative Commons Attribution. 1MB |
Abstract
Lamina I of the dorsal horn, together with its main output pathway, lamina I projection neurons, has long been implicated in the processing of nociceptive stimuli, as well as the development of chronic pain conditions. However, the study of lamina I projection neurons is hampered by technical challenges, including the low throughput and selection biases of traditional electrophysiological techniques. Here we report on a technique that uses anatomical labelling strategies and in vivo imaging to simultaneously study a network of lamina I projection neurons in response to electrical and natural stimuli. Although we were able to confirm the nociceptive involvement of this group of cells, we also describe an unexpected preference for innocuous cooling stimuli. We were able to characterize the thermal responsiveness of these cells in detail and found cooling responses decline when exposed to stable cold temperatures maintained for more than a few seconds, as well as to encode the intensity of the end temperature, while heating responses showed an unexpected reliance on adaptation temperatures.
Item Type: | Articles |
---|---|
Additional Information: | This project was supported by a strategic award from the Wellcome Trust (102645/Z/13/Z). |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Gutierrez-Mecinas, Dr Maria and Beresford-Polgar, Dr Erika and Todd, Professor Andrew |
Creator Roles: | Polgár, E.Methodology, Writing – original draft Gutierrez-Mecinas, M.Methodology, Writing – original draft Todd, A. J.Writing – original draft |
Authors: | Chisholm, K. I., Lo Re, L., Polgár, E., Gutierrez-Mecinas, M., Todd, A. J., and McMahon, S. B. |
College/School: | College of Medical Veterinary and Life Sciences > School of Psychology & Neuroscience |
Journal Name: | Pain |
Publisher: | Lippincott, Williams & Wilkins |
ISSN: | 0304-3959 |
ISSN (Online): | 1872-6623 |
Published Online: | 24 March 2021 |
Copyright Holders: | Copyright © 2021 The Authors |
First Published: | First published in Pain 162(9): 2405-2417 |
Publisher Policy: | Reproduced under a Creative Commons License |
University Staff: Request a correction | Enlighten Editors: Update this record