Human cytomegalovirus genome diversity in longitudinally collected breast milk samples

Götting, J., Lazar, K., Suárez, N. M., Steinbrück, L., Rabe, T., Goelz, R., Schulz, T. F., Davison, A. J. , Hamprecht, K. and Ganzenmueller, T. (2021) Human cytomegalovirus genome diversity in longitudinally collected breast milk samples. Frontiers in Cellular and Infection Microbiology, 11, 664247. (doi: 10.3389/fcimb.2021.664247) (PMID:33937103) (PMCID:PMC8085339)

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Abstract

Reactivation and shedding of human cytomegalovirus (HCMV) in breast milk during lactation is highly frequent in HCMV-seropositive mothers. This represents a key transmission route for postnatal HCMV infection and can lead to severe disease in preterm neonates. Little is known about HCMV strain composition or longitudinal intrahost viral population dynamics in breast milk from immunocompetent women. We performed HCMV-specific target enrichment and high-throughput sequencing of 38 breast milk samples obtained in Germany between days 10 and 60 postpartum from 15 mothers with HCMV DNA-lactia, and assembled HCMV consensus sequences de novo. The genotype distribution and number of HCMV strains present in each sample were determined by quantifying genotype-specific sequence motifs in 12 hypervariable viral genes, revealing a wide range of genotypes (82/109) for these genes in the cohort and a unique, longitudinally stable strain composition in each mother. Reactivation of up to three distinct HCMV strains was detected in 8/15 of mothers, indicating that a representative subset of the woman’s HCMV reservoir might be locally reactivated early during lactation. As described previously, nucleotide diversity of samples with multiple strains was much higher than that of samples with single strains. Breast milk as a main source of postnatal mother-to-infant transmission may serve as a repository for viral diversity and thus play an essential role in the natural epidemiology of HCMV.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Suarez, Dr Nicolas and Davison, Professor Andrew
Authors: Götting, J., Lazar, K., Suárez, N. M., Steinbrück, L., Rabe, T., Goelz, R., Schulz, T. F., Davison, A. J., Hamprecht, K., and Ganzenmueller, T.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Frontiers in Cellular and Infection Microbiology
Publisher:Frontiers Media
ISSN:2235-2988
ISSN (Online):2235-2988
Copyright Holders:Copyright © 2021 Götting, Lazar, Suarez, Steinbrück, Rabe, Goelz, Schulz, Davison, ́ Hamprecht and Ganzenmueller
First Published:First published in Frontiers in Cellular and Infection Microbiology 11: 664247
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
656321Genomics of human cytomegalovirusAndrew DavisonMedical Research Council (MRC)MC_UU_12014/3MVLS III - CENTRE FOR VIRUS RESEARCH