Butcher, M. C., Brown, J. L. , Hansom, D., Wilson-van Os, R., Delury, C., Laycock, P. A. and Ramage, G. (2021) Assessing the bioactive profile of anti-fungal loaded calcium sulfate against fungal biofilms. Antimicrobial Agents and Chemotherapy, 65(6), e02551-20. (doi: 10.1128/AAC.02551-20) (PMID:33753336)
Text
236535.pdf - Published Version Available under License Creative Commons Attribution. 2MB |
Abstract
Calcium sulfate (CS) has been used clinically as a bone or void filling biomaterial, and due to its resorptive properties have provided the prospect for its use as a release mechanism for local antibiotics to control biofilms. Here, we aimed to test CS beads loaded with three antifungal drugs against planktonic and sessile fungal species to assess whether these antifungal beads could be harnessed to provide consistent release of antifungals at biofilm inhibitive doses. A panel of different fungal species (n=15) were selected for planktonic broth microdilution testing with fluconazole (FLZ), amphotericin B (AMB) and caspofungin (CSP). After establishing planktonic inhibition, antifungal CS beads were introduced to fungal biofilms (n=5) to assess biofilm formation and cell viability through a combination of standard quantitative and qualitative biofilm assays. Inoculation of a hydrogel substrate, packed with antifungal CS beads, was also used to assess diffusion through a semi-dry material, to mimic active infection in-vivo. In general, antifungals released from CS loaded beads were all effective at inhibiting the pathogenic fungi over 7-days within standard MIC ranges for these fungi. We observed a significant reduction of pre-grown fungal biofilms across key fungal pathogens following treatment, with visually observable changes in cell morphology and biofilm coverage provided by scanning electron microscopy. Assessment of biofilm inhibition also revealed reductions in total and viable cells across all organisms tested. These data show that antifungal loaded CS beads produce a sustained antimicrobial effect, which inhibits and kills clinically relevant fungal species in-vitro as planktonic and biofilm cells.
Item Type: | Articles |
---|---|
Additional Information: | The authors would like to acknowledge funding support from Biocomposites for MB and GR. |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Butcher, Mr Mark and Ramage, Professor Gordon and Hansom, Mr Donald and Brown, Dr Jason |
Authors: | Butcher, M. C., Brown, J. L., Hansom, D., Wilson-van Os, R., Delury, C., Laycock, P. A., and Ramage, G. |
College/School: | College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School |
Journal Name: | Antimicrobial Agents and Chemotherapy |
Publisher: | American Society for Microbiology |
ISSN: | 0066-4804 |
ISSN (Online): | 1098-6596 |
Published Online: | 22 March 2021 |
Copyright Holders: | Copyright © 2021 Butcher et al. |
First Published: | First published in Antimicrobial Agents and Chemotherapy 65(6): e02551-20 |
Publisher Policy: | Reproduced under a Creative Commons license |
University Staff: Request a correction | Enlighten Editors: Update this record