Stratum recruits Rab8 at Golgi exit sites to regulate the basolateral sorting of Notch and Sanpodo

Bellec, K. , Gicquel, I. and Le Borgne, R. (2018) Stratum recruits Rab8 at Golgi exit sites to regulate the basolateral sorting of Notch and Sanpodo. Development, 145(13), dev163469. (doi: 10.1242/dev.163469) (PMID:29967125)

Abstract

In Drosophila, the sensory organ precursor (SOP or pI cell) divides asymmetrically to give birth to daughter cells, the fates of which are governed by the differential activation of the Notch pathway. Proteolytic activation of Notch induced by ligand is based on the correct polarized sorting and localization of the Notch ligand Delta, the Notch receptor and its trafficking partner Sanpodo (Spdo). Here, we have identified Stratum (Strat), a presumptive guanine nucleotide exchange factor for Rab GTPases, as a regulator of Notch activation. Loss of Strat causes cell fate transformations associated with an accumulation of Notch, Delta and Spdo in the trans-Golgi network (TGN), and an apical accumulation of Spdo. The strat mutant phenotype is rescued by the catalytically active as well as the wild-type form of Rab8, suggesting a chaperone function for Strat rather than that of exchange factor. Strat is required to localize Rab8 at the TGN, and rab8 phenocopies strat. We propose that Strat and Rab8 act at the exit of the Golgi apparatus to regulate the sorting and the polarized distribution of Notch, Delta and Spdo.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Bellec, Miss Karen

Creator Roles

Bellec, K.: Conceptualization, Methodology, Formal analysis, Investigation, Resources, Writing – original draft, Writing – review and editing, Visualization

• Authors: Bellec, K., Gicquel, I., and Le Borgne, R.
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
• Journal Name: Development
• Publisher: Company of Biologists
• ISSN: 0950-1991
• ISSN (Online): 1477-9129
• Published Online: 02 July 2018
• Copyright Holders: Copyright © 2018 The Company of Biologists Ltd
• First Published: First published in Development 145(13): dev163469
• Publisher Policy: Reproduced in accordance with the publisher copyright policy