The clathrin adaptor AP-1 and Stratum act in parallel pathways to control Notch activation in Drosophila sensory organ precursors cells

Bellec, K. , Pinot, M., Gicquel, I. and Le Borgne, R. (2021) The clathrin adaptor AP-1 and Stratum act in parallel pathways to control Notch activation in Drosophila sensory organ precursors cells. Development, 148(1), dev191437. (doi: 10.1242/dev.191437) (PMID:33298463) (PMCID:PMC7823167)

Abstract

Drosophila sensory organ precursors divide asymmetrically to generate pIIa/pIIb cells, the identity of which relies on activation of Notch at cytokinesis. Although Notch is present apically and basally relative to the midbody at the pIIa-pIIb interface, the basal pool of Notch is reported to be the main contributor for Notch activation in the pIIa cell. Intra-lineage signalling requires appropriate apico-basal targeting of Notch, its ligand Delta and its trafficking partner Sanpodo. We have previously reported that AP-1 and Stratum regulate the trafficking of Notch and Sanpodo from the trans-Golgi network to the basolateral membrane. Loss of AP-1 or Stratum caused mild Notch gain-of-function phenotypes. Here, we report that their concomitant loss results in a penetrant Notch gain-of-function phenotype, indicating that they control parallel pathways. Although unequal partitioning of cell fate determinants and cell polarity were unaffected, we observed increased amounts of signalling-competent Notch as well as Delta and Sanpodo at the apical pIIa-pIIb interface, at the expense of the basal pool of Notch. We propose that AP-1 and Stratum operate in parallel pathways to localize Notch and control where receptor activation takes place.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Bellec, Miss Karen

Creator Roles

Bellec, K.: Conceptualization, Methodology, Validation, Formal analysis, Investigation, Resources, Writing – original draft, Writing – review and editing, Visualization

• Authors: Bellec, K., Pinot, M., Gicquel, I., and Le Borgne, R.
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
• Journal Name: Development
• Publisher: Company of Biologists
• ISSN: 0950-1991
• ISSN (Online): 1477-9129
• Published Online: 09 December 2020
• Copyright Holders: Copyright © 2021 The Company of Biologists Ltd
• First Published: First published in Development 148(1): dev191437
• Publisher Policy: Reproduced under a Creative Commons License