First steps towards effective methods in exploiting high-throughput technologies for the determination of human protein structures of high biomedical value

Banci, L. et al. (2006) First steps towards effective methods in exploiting high-throughput technologies for the determination of human protein structures of high biomedical value. Acta Crystallographica. Section D: Biological Crystallography, 62(10), pp. 1208-1217. (doi: 10.1107/S0907444906029350)

Abstract

The EC 'Structural Proteomics In Europe' contract is aimed specifically at the atomic resolution structure determination of human protein targets closely linked to health, with a focus on cancer ( kinesins, kinases, proteins from the ubiquitin pathway), neurological development and neurodegenerative diseases and immune recognition. Despite the challenging nature of the analysis of such targets, similar to 170 structures have been determined to date. Here, the impact of high- throughput technologies, such as parallel expression of multiple constructs, the use of standardized refolding protocols and optimized crystallization screens or the use of mass spectrometry to assist sample preparation, on the structural biology of mammalian protein targets is illustrated through selected examples.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Kozielski, Professor Frank
• Authors: Banci, L., Bertini, I., Cusack, S., de Jong, R., Heinemann, U., Jones, E., Kozielski, F., Maskos, K., Messerschmidt, A., Owens, R., Perrakis, A., Poterszman, A., Schneider, G., Siebold, C., Silman, I., Sixma, T., Stewart-Jones, G., Sussman, J., Thierry, J., and Moras, D.
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
• Journal Name: Acta Crystallographica. Section D: Biological Crystallography
• Publisher: Wiley-Blackwell Publishing, Inc.
• ISSN: 0907-4449
• ISSN (Online): 1399-0047