Bacillus subtilis RarA acts as a positive RecA accessory protein

Romero, H., Serrano, E. , Hernández-Tamayo, R., Carrasco, B., Cárdenas, P. P., Ayora, S., Graumann, P. L. and Alonso, J. C. (2020) Bacillus subtilis RarA acts as a positive RecA accessory protein. Frontiers in Microbiology, 11, 92. (doi: 10.3389/fmicb.2020.00092) (PMID:32117122) (PMCID:PMC7031210)

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Abstract

Ubiquitous RarA AAA+ ATPases play crucial roles in the cellular response to blocked replication forks in pro- and eukaryotes. Here, we provide evidence that absence of RarA reduced the viability of ΔrecA, ΔrecO, and recF15 cells during unperturbed growth. The rarA gene was epistatic to recO and recF genes in response to H2O2- or MMS-induced DNA damage. Conversely, the inactivation of rarA partially suppressed the HR defect of mutants lacking end-resection (ΔaddAB, ΔrecJ, ΔrecQ, ΔrecS) or branch migration (ΔruvAB, ΔrecG, ΔradA) activity. RarA contributes to RecA thread formation, that are thought to be the active forms of RecA during homology search. The absence of RarA reduced RecA accumulation, and the formation of visible RecA threads in vivo upon DNA damage. When ΔrarA was combined with mutations in genuine RecA accessory genes, RecA accumulation was further reduced in ΔrarA ΔrecU and ΔrarA ΔrecX double mutant cells, and was blocked in ΔrarA recF15 cells. These results suggest that RarA contributes to the assembly of RecA nucleoprotein filaments onto single-stranded DNA, and possibly antagonizes RecA filament disassembly.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Serrano, Dr Ester
Authors: Romero, H., Serrano, E., Hernández-Tamayo, R., Carrasco, B., Cárdenas, P. P., Ayora, S., Graumann, P. L., and Alonso, J. C.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:Frontiers in Microbiology
Publisher:Frontiers Media
ISSN:1664-302X
ISSN (Online):1664-302X
Copyright Holders:Copyright © 2020 Romero, Serrano, Hernández-Tamayo, Carrasco, Cárdenas, Ayora, Graumann and Alonso
First Published:First published in Frontiers in Microbiology 11: 92
Publisher Policy:Reproduced under a Creative Commons License

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