Detection of genotype-specific antibody responses to glycoproteins B and H in primary and non-primary human cytomegalovirus infections by peptide-based ELISA

Zavaglio, F., Fiorina, L., Suarez, N. M., Fornara, C., De Cicco, M., Cirasola, D., Davison, A. J. , Gerna, G. and Lilleri, D. (2021) Detection of genotype-specific antibody responses to glycoproteins B and H in primary and non-primary human cytomegalovirus infections by peptide-based ELISA. Viruses, 13(3), 399. (doi: 10.3390/v13030399) (PMID:33802390) (PMCID:PMC7998382)

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Abstract

Background: Strain-specific antibodies to human cytomegalovirus (HCMV) glycoproteins B and H (gB and gH) have been proposed as a potential diagnostic tool for identifying reinfection. We investigated genotype-specific IgG antibody responses in parallel with defining the gB and gH genotypes of the infecting viral strains. Methods: Subjects with primary (n = 20) or non-primary (n = 25) HCMV infection were studied. The seven gB (gB1-7) and two gH (gH1-2) genotypes were determined by real-time PCR and whole viral genome sequencing, and genotype-specific IgG antibodies were measured by a peptide-based enzyme-linked immunosorbent assay (ELISA). Results: Among subjects with primary infection, 73% (n = 8) infected by gB1-HCMV and 63% (n = 5) infected by gB2/3-HCMV had genotype-specific IgG antibodies to gB (gB2 and gB3 are similar in the region tested). Peptides from the rarer gB4-gB7 genotypes had nonspecific antibody responses. All subjects infected by gH1-HCMV and 86% (n = 6/7) infected by gH2-HCMV developed genotype-specific responses. Among women with non-primary infection, gB and gH genotype-specific IgG antibodies were detected in 40% (n = 10) and 80% (n = 20) of subjects, respectively. Conclusions: Peptide-based ELISA is capable of detecting primary genotype-specific IgG responses to HCMV gB and gH, and could be adopted for identifying reinfections. However, about half of the subjects did not have genotype-specific IgG antibodies to gB.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Suarez, Dr Nicolas and Davison, Professor Andrew
Authors: Zavaglio, F., Fiorina, L., Suarez, N. M., Fornara, C., De Cicco, M., Cirasola, D., Davison, A. J., Gerna, G., and Lilleri, D.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Viruses
Publisher:MDPI
ISSN:1999-4915
ISSN (Online):1999-4915
Published Online:03 March 2021
Copyright Holders:Copyright © 2021 The Authors
First Published:First published in Viruses 13(3): 399
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
656321Genomics of human cytomegalovirusAndrew DavisonMedical Research Council (MRC)MC_UU_12014/3MVLS III - CENTRE FOR VIRUS RESEARCH