Amphiphilic porphyrins for second harmonic generation imaging

Reeve, J. E., Collins, H. A., De Mey, K., Kohl, M. M. , Thorley, K. J., Paulsen, O., Clays, K. and Anderson, H. L. (2009) Amphiphilic porphyrins for second harmonic generation imaging. Journal of the American Chemical Society, 131(8), pp. 2758-2759. (doi: 10.1021/ja8061369) (PMID:19209855)

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Abstract

Amphiphilic donor−acceptor meso-ethynyl porphyrins with polar pyridinium electron-acceptor head groups and hydrophobic dialkyl-aniline electron donors have high molecular hyperpolarizabilities (as measured by hyper-Rayleigh scattering) and high affinities for biological membranes. When bound to water droplets in dodecane, or to the plasma membranes of living cells, they can be used for second harmonic generation (SHG) microscopy; an incident light of wavelength 840 nm generates a strong frequency-doubled signal at 420 nm. Copper(II) and nickel(II) porphyrin complexes give similar SHG signals to those of the free-base porphyrins, while exhibiting no detectable two-photon excited fluorescence.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Kohl, Dr Michael
Authors: Reeve, J. E., Collins, H. A., De Mey, K., Kohl, M. M., Thorley, K. J., Paulsen, O., Clays, K., and Anderson, H. L.
College/School:College of Medical Veterinary and Life Sciences > Institute of Neuroscience and Psychology
Journal Name:Journal of the American Chemical Society
Publisher:American Chemical Society
ISSN:0002-7863
ISSN (Online):1520-5126
Published Online:11 February 2009

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