Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates

Botcherby, E. J., Smith, C. W., Kohl, M. M. , Débarre, D., Booth, M. J., Juškaitis, R., Paulsen, O. and Wilson, T. (2012) Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates. Proceedings of the National Academy of Sciences of the United States of America, 109(8), pp. 2919-2924. (doi: 10.1073/pnas.1111662109) (PMID:22315405) (PMCID:PMC3286923)

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Abstract

Multiphoton microscopy is a powerful tool in neuroscience, promising to deliver important data on the spatiotemporal activity within individual neurons as well as in networks of neurons. A major limitation of current technologies is the relatively slow scan rates along the z direction compared to the kHz rates obtainable in the x and y directions. Here, we describe a custom-built microscope system based on an architecture that allows kHz scan rates over hundreds of microns in all three dimensions without introducing aberration. We further demonstrate how this high-speed 3D multiphoton imaging system can be used to study neuronal activity at millisecond resolution at the subcellular as well as the population level.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Kohl, Dr Michael
Authors: Botcherby, E. J., Smith, C. W., Kohl, M. M., Débarre, D., Booth, M. J., Juškaitis, R., Paulsen, O., and Wilson, T.
College/School:College of Medical Veterinary and Life Sciences > Institute of Neuroscience and Psychology
Journal Name:Proceedings of the National Academy of Sciences of the United States of America
Publisher:National Academy of Sciences
ISSN:0027-8424
ISSN (Online):1091-6490
Published Online:06 February 2012

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