A hydrogel platform that incorporates laminin isoforms for efficient presentation of growth factors – neural growth and osteogenesis

Dobre, O. et al. (2021) A hydrogel platform that incorporates laminin isoforms for efficient presentation of growth factors – neural growth and osteogenesis. Advanced Functional Materials, 31(21), 2010225. (doi: 10.1002/adfm.202010225)

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Abstract

Laminins (LMs) are important structural proteins of the extracellular matrix (ECM). The abundance of every LM isoform is tissue‐dependent, suggesting that LM has tissue‐specific roles. LM binds growth factors (GFs), which are powerful cytokines widely used in tissue engineering due to their ability to control stem cell differentiation. Currently, the most commonly used ECM mimetic material in vitro is Matrigel, a matrix of undefined composition containing LM and various GFs, but subjected to batch variability and lacking control of physicochemical properties. Inspired by Matrigel, a new and completely defined hydrogel platform based on hybrid LM‐poly(ethylene glycol) (PEG) hydrogels with controllable stiffness (1–25 kPa) and degradability is proposed. Different LM isoforms are used to bind and efficiently display GFs (here, bone morphogenetic protein (BMP‐2) and beta‐nerve growth factor (β‐NGF)), enabling their solid‐phase presentation at ultralow doses to specifically target a range of tissues. The potential of this platform to trigger stem cell differentiation toward osteogenic lineages and stimulate neural cells growth in 3D, is demonstrated. These hydrogels enable 3D, synthetic, defined composition, and reproducible cell culture microenvironments reflecting the complexity of the native ECM, where GFs in combination with LM isoforms yield the full diversity of cellular processes.

Item Type:Articles
Additional Information:The authors acknowledge support via an EPSRC Programme Grant (EP/P001114/1).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Salmeron-Sanchez, Professor Manuel and CICCONE, Mr GIUSEPPE and Dalby, Professor Matthew and Llopis-Hernandez, Dr Virginia and Rodrigo-Navarro, Mr Aleixandre and Dobre, Dr Oana and Vassalli, Dr Massimo and VENTERS, Douglas and Gonzalez Garcia, Dr Cristina and Trujillo-Muñoz, Sara
Authors: Dobre, O., Oliva, M. A.G., Ciccone, G., Trujillo, S., Rodrigo-Navarro, A., Venters, D. C., Llopis-Hernandez, V., Vassalli, M., Gonzalez Garcia, C., Dalby, M. J., and Salmeron-Sanchez, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Advanced Functional Materials
Publisher:Wiley
ISSN:1616-301X
ISSN (Online):1616-301X
Published Online:21 March 2021
Copyright Holders:Copyright © 2021 The Authors
First Published:First published in Advanced Functional Materials 31(21): 2010225
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
173192Engineering growth factor microenvironments- a new therapeutic paradigm for regenerative medicineManuel Salmeron-SanchezEngineering and Physical Sciences Research Council (EPSRC)EP/P001114/1ENG - Biomedical Engineering